PMID- 9820197
OWN - NLM
STAT- MEDLINE
DCOM- 19990205
LR  - 20101118
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 51
IP  - 4
DP  - 1998 Dec
TI  - Rapid detection of male-specific DNA sequence in bovine embryos using 
      fluorescence in situ hybridization.
PG  - 390-4
AB  - An accurate, reliable, and quick (less than an hour) method for determining the 
      sex of bovine embryos was developed using a fluorescence in situ hybridization 
      (FISH), with a probe designed from a bovine Y chromosome specific DNA (BC1.2). 
      First, to improve a protocol of FISH and evaluate an accuracy of the method, 
      lymphocyte nuclei prepared from three bulls, two cows, and one freemartin were 
      tested. We found that 5 min was enough for hybridization. The washing solution 
      adequate for posthybridization was 0.5x SSC at 72 degrees C for 5 min. The whole 
      procedure for FISH can be accomplished in less than an hour. A male-specific 
      signal was detected, on average, as 97, 0.5, and 83%, respectively, of 
      lymphocytes in males, females, and a freemartin. Using the rapid FISH protocol 
      developed, 28 embryos were divided. According to the presence of the digoxigenin 
      signal, 16 embryos (57.1%) were predicted as male, and 12 embryos (42.9%), 
      predicted as female.
FAU - Kobayashi, J
AU  - Kobayashi J
AD  - Miyagi Agricultural College, Sendai, Japan.
FAU - Sekimoto, A
AU  - Sekimoto A
FAU - Uchida, H
AU  - Uchida H
FAU - Wada, T
AU  - Wada T
FAU - Sasaki, K
AU  - Sasaki K
FAU - Sasada, H
AU  - Sasada H
FAU - Umezu, M
AU  - Umezu M
FAU - Sato, E
AU  - Sato E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Nucleus/chemistry
MH  - DNA/*chemistry
MH  - Embryo, Mammalian/*chemistry
MH  - Female
MH  - Freemartinism/genetics
MH  - In Situ Hybridization, Fluorescence/methods/*veterinary
MH  - Lymphocytes/chemistry
MH  - Male
MH  - Sequence Analysis, DNA/methods/veterinary
MH  - *Sex Characteristics
MH  - Sex Determination Analysis/*veterinary
MH  - Sex Determination Processes
MH  - Y Chromosome
EDAT- 1998/11/20 06:38
MHDA- 2000/06/20 09:00
CRDT- 1998/11/20 06:38
PHST- 1998/11/20 06:38 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/11/20 06:38 [entrez]
AID - 10.1002/(SICI)1098-2795(199812)51:4<390::AID-MRD5>3.0.CO;2-F [pii]
AID - 10.1002/(SICI)1098-2795(199812)51:4<390::AID-MRD5>3.0.CO;2-F [doi]
PST - ppublish
SO  - Mol Reprod Dev. 1998 Dec;51(4):390-4. doi: 
      10.1002/(SICI)1098-2795(199812)51:4<390::AID-MRD5>3.0.CO;2-F.