PMID- 9821164
OWN - NLM
STAT- MEDLINE
DCOM- 19990121
LR  - 20190921
IS  - 0305-1846 (Print)
IS  - 0305-1846 (Linking)
VI  - 24
IP  - 5
DP  - 1998 Oct
TI  - Expression of amyloid precursor protein (beta-APP) in the neonatal brain 
      following hypoxic ischaemic injury.
PG  - 346-52
AB  - Perinatal hypoxic ischaemic brain injury (HII) is a major cause of neonatal 
      mortality and long-term neurological morbidity. An understanding of the molecular 
      events which follow HII may lead to novel treatments to improve the final outcome 
      for affected infants. The beta-amyloid precursor protein (beta-APP) is a widely 
      expressed transmembrane protein whose proposed functions include stabilization of 
      neuronal calcium fluxes, inhibition of the clotting cascade and cell-cell or 
      cell-matrix adhesion. Normally present at low levels in neurons its expression is 
      induced as part of the acute response of the adult brain to HII. This study aimed 
      to determine whether beta-APP is also part of the acute adaptive response of the 
      infant brain to HII. Immunohistochemistry and Western blotting were used to 
      assess cerebral beta-APP expression in 14-day-old rat pups subjected to 
      unilateral HII, and in 10 term human infants, who died between 12 h and 16 months 
      after severe perinatal HII. In the rat pups beta-APP expression was increased by 
      2 h post-injury, peaked, fourfold above control levels, at 24 h and gradually 
      declined over the following 4 days. Expression was induced bilaterally, but was 
      greater on the side of injury. In the human infants, increased, predominantly 
      neuronal expression of beta-APP, was detectable immunohistochemically within 24 h 
      of injury and was greatest in those infants dying within 3 days. Expression was 
      particularly strong in the areas showing histological evidence of injury, but was 
      also seen in apparently undamaged areas. We conclude that beta-APP induction is 
      part of the the acute adaptive response of the neonatal brain to HII.
FAU - Baiden-Amissah, K
AU  - Baiden-Amissah K
AD  - Division of Investigative Science, Imperial College School of Medicine, 
      Hammersmith Hospital, London, UK.
FAU - Joashi, U
AU  - Joashi U
FAU - Blumberg, R
AU  - Blumberg R
FAU - Mehmet, H
AU  - Mehmet H
FAU - Edwards, A D
AU  - Edwards AD
FAU - Cox, P M
AU  - Cox PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuropathol Appl Neurobiol
JT  - Neuropathology and applied neurobiology
JID - 7609829
RN  - 0 (Amyloid beta-Protein Precursor)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*analysis/*biosynthesis
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Western
MH  - Brain Chemistry/*physiology
MH  - Brain Ischemia/*metabolism
MH  - Disease Models, Animal
MH  - Humans
MH  - Hypoxia, Brain/*metabolism
MH  - Immunohistochemistry
MH  - Rats
EDAT- 1998/11/20 00:00
MHDA- 1998/11/20 00:01
CRDT- 1998/11/20 00:00
PHST- 1998/11/20 00:00 [pubmed]
PHST- 1998/11/20 00:01 [medline]
PHST- 1998/11/20 00:00 [entrez]
AID - 10.1046/j.1365-2990.1998.00141.x [doi]
PST - ppublish
SO  - Neuropathol Appl Neurobiol. 1998 Oct;24(5):346-52. doi: 
      10.1046/j.1365-2990.1998.00141.x.