PMID- 9821867
OWN - NLM
STAT- MEDLINE
DCOM- 19990120
LR  - 20071115
IS  - 0001-527X (Print)
IS  - 0001-527X (Linking)
VI  - 45
IP  - 2
DP  - 1998
TI  - Contents of total and protein-bound carbohydrates are low in leukemic leukocytes 
      from patients with acute myelogenous leukemia.
PG  - 361-71
AB  - Leukemic leukocytes from 12 patients with acute myelogenous leukemia (AML) and 
      two patients with chronic myelogenous leukemia (CML) were isolated by 
      centrifugations in Percoll gradients, and examined for total carbohydrates. In 
      leukemic leukocytes from 10 of these patients ceramide-bound carbohydrates were 
      also determined. Protein-bound carbohydrates were calculated by subtraction of 
      ceramide-bound carbohydrates from total carbohydrates. In all samples analysed 
      the contents of total and protein-bound carbohydrates were much lower in leukemic 
      leukocytes than in normal neutrophils, irrespective whether the results were 
      expressed relative to protein, DNA, cell number or dry mass. For immature 
      leukemic cells of M0-M1 phenotype differences up to 10-fold were observed. 
      Contents of ceramide-bound carbohydrates, i.e. those of neutral and acidic 
      glycosphingolipids (GSLs) were also low in leukemic cells. However, when GSL 
      carbohydrates were calculated as percentage of total carbohydrates, GSLs in 
      leukemic leukocytes were elevated in half of the AML patients but depressed in 
      the other half. The results are discussed in the light of the hypothesis on GSL 
      function by one of us (Koscielak J., 1986, Glycoconjugate J. 3, 95-108). 
      According to one element of the hypothesis, during cell differentiation newly 
      synthesized glycoproteins (GPs) that perform specific functions are added to 
      house-keeping GPs that are present in plasma membranes of all types of cells. 
      Thus, during differentiation, the GP content of the cell membrane should increase 
      and that of the so called "membrane packing" glycosphingolipids should decrease.
FAU - Smolenska-Sym, G
AU  - Smolenska-Sym G
AD  - Department of Biochemistry, Institute of Hematology and Blood Transfusion, 
      Warsaw, Poland.
FAU - Zdebska, E
AU  - Zdebska E
FAU - Golaszewska, E
AU  - Golaszewska E
FAU - Wozniak, J
AU  - Wozniak J
FAU - Durzynski, T
AU  - Durzynski T
FAU - Maj, S
AU  - Maj S
FAU - Mokras, U
AU  - Mokras U
FAU - Koscielak, J
AU  - Koscielak J
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Acta Biochim Pol
JT  - Acta biochimica Polonica
JID - 14520300R
RN  - 0 (Carbohydrates)
RN  - 0 (Proteins)
SB  - IM
MH  - *Carbohydrate Metabolism
MH  - Carbohydrates/chemistry
MH  - Cell Transformation, Neoplastic
MH  - Humans
MH  - Leukemia, Myeloid, Acute/*blood/pathology
MH  - Leukocytes/*metabolism/pathology
MH  - Protein Binding
MH  - Proteins
EDAT- 1998/11/20 00:00
MHDA- 1998/11/20 00:01
CRDT- 1998/11/20 00:00
PHST- 1998/11/20 00:00 [pubmed]
PHST- 1998/11/20 00:01 [medline]
PHST- 1998/11/20 00:00 [entrez]
PST - ppublish
SO  - Acta Biochim Pol. 1998;45(2):361-71.