PMID- 9822290
OWN - NLM
STAT- MEDLINE
DCOM- 19981214
LR  - 20240510
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 114
IP  - 2
DP  - 1998 Nov
TI  - Scleroderma fibroblasts promote migration of mononuclear leucocytes across 
      endothelial cell monolayers.
PG  - 293-300
AB  - Perivascular infiltrates of inflammatory cells are a hallmark of lesional skin in 
      scleroderma. We have explored the potential for scleroderma fibroblasts to 
      modulate mononuclear leucocyte migration across endothelial cell monolayers in 
      tissue culture, and to regulate expression of endothelial cell adhesion 
      molecules. Fibroblasts were grown from skin biopsies of eight patients with 
      active diffuse cutaneous scleroderma and from four healthy controls. Co-culture 
      and conditioned medium transfer experiments examined the effect of soluble 
      fibroblast products on mononuclear leucocyte (U937) cell migration across 
      endothelial cell (1E-7) monolayers grown on tissue culture inserts. Co-culture of 
      scleroderma, but not control fibroblasts, promoted transendothelial migration of 
      U937 cells. Scleroderma fibroblast-conditioned medium had qualitatively similar 
      effects and equivalent results were obtained using Jurkat-6 (T lymphocyte) cells, 
      and with peripheral blood mononuclear cells from a patient with diffuse cutaneous 
      scleroderma. Promotion of leucocyte migration does not appear to result from 
      increased endothelial adhesion molecule expression, since fibroblast-conditioned 
      medium did not up-regulate endothelial cell expression of intercellular adhesion 
      molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) or E-selectin. 
      Moreover, leucocyte migration across cytokine-activated endothelial cell layers 
      in co-culture with fibroblasts was less than across resting cells, although the 
      selective effect of scleroderma fibroblast co-culture persisted. Recombinant 
      monocyte chemoattractant protein-1 (MCP-1) or IL-8 increased passage of 
      mononuclear leucocytes across endothelial cell monolayers, whilst anti-MCP-1, but 
      not anti-IL-8 antibodies, significantly reduced the effect of fibroblast 
      conditioned medium. These data suggest that systemic sclerosis (SSc) fibroblasts 
      promote leucocyte migration across endothelial cell monolayers in tissue culture 
      via an MCP-1-dependent mechanism. These findings may be relevant to the 
      perivascular mononuclear leucocyte infiltrates characteristic of early SSc 
      lesions.
FAU - Denton, C P
AU  - Denton CP
AD  - Vascular Biology Research Centre, King's College London, Academic Unit of 
      Rheumatology, Royal Free Hospital, UK.
FAU - Shi-Wen, X
AU  - Shi-Wen X
FAU - Sutton, A
AU  - Sutton A
FAU - Abraham, D J
AU  - Abraham DJ
FAU - Black, C M
AU  - Black CM
FAU - Pearson, J D
AU  - Pearson JD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Cell Line, Transformed
MH  - *Chemotaxis, Leukocyte
MH  - Endothelium, Vascular/*pathology
MH  - Female
MH  - Fibroblasts/cytology/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/biosynthesis
MH  - Jurkat Cells
MH  - Leukocytes, Mononuclear/*physiology
MH  - Male
MH  - Middle Aged
MH  - Scleroderma, Localized/immunology/*pathology
MH  - U937 Cells
MH  - Vascular Cell Adhesion Molecule-1/biosynthesis
PMC - PMC1905096
EDAT- 1998/11/20 00:00
MHDA- 1998/11/20 00:01
PMCR- 1999/11/01
CRDT- 1998/11/20 00:00
PHST- 1998/11/20 00:00 [pubmed]
PHST- 1998/11/20 00:01 [medline]
PHST- 1998/11/20 00:00 [entrez]
PHST- 1999/11/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2249.1998.00721.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1998 Nov;114(2):293-300. doi: 10.1046/j.1365-2249.1998.00721.x.