PMID- 9822987
OWN - NLM
STAT- MEDLINE
DCOM- 19990202
LR  - 20220318
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 15
IP  - 9
DP  - 1998 Oct
TI  - Improving the fixation method for preimplantation genetic diagnosis by 
      fluorescent in situ hybridization.
PG  - 570-4
AB  - PURPOSE: Our purpose was to modify a fixation method using Tween-20 and HCl (TH) 
      and to compare it with a protocol using methanol and acetic acid (MA) for the 
      improvement of preimplantation genetic diagnosis by fluorescence in situ 
      hybridization (FISH). METHODS: Single blastomeres were allocated to either the TH 
      or the MA procedure. The two methods were compared to evaluate efficiency of 
      fixation and the intensities of FISH signals. RESULTS: With the TH method, 123 
      (93.9%) of 131 blastomeres were fixed, while only 95 (78.5%) of 121 were fixed 
      with MA. Average scores for the intensity of FISH signals were significantly 
      stronger for TH than for MA (P < 0.05). There was also a significant difference 
      in signal intensity scores between the two methods for type-3 nuclei. 
      CONCLUSIONS: Our results indicate that not only are fewer nuclei lost during 
      fixation but also stronger FISH signals can be obtained with the TH method. Thus, 
      modified TH can improve the overall efficiency of preimplantation genetic 
      diagnosis.
FAU - Xu, K
AU  - Xu K
AD  - Center for Reproductive Medicine and Infertility, Cornell University Medical 
      College, New York, New York 10021, USA.
FAU - Huang, T
AU  - Huang T
FAU - Liu, T
AU  - Liu T
FAU - Shi, Z
AU  - Shi Z
FAU - Rosenwaks, Z
AU  - Rosenwaks Z
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (DNA Probes)
RN  - 0 (Polysorbates)
RN  - 0 (Surface-Active Agents)
RN  - Q40Q9N063P (Acetic Acid)
RN  - QTT17582CB (Hydrochloric Acid)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Acetic Acid/chemistry
MH  - Blastomeres/*physiology
MH  - DNA Probes/chemistry
MH  - Female
MH  - Humans
MH  - Hydrochloric Acid/chemistry
MH  - Image Processing, Computer-Assisted
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Methanol/chemistry
MH  - Polysorbates/chemistry
MH  - Pregnancy
MH  - Preimplantation Diagnosis/*methods
MH  - Random Allocation
MH  - Surface-Active Agents/chemistry
MH  - Tissue Fixation/*methods
PMC - PMC3454918
EDAT- 1998/11/21 00:00
MHDA- 1998/11/21 00:01
PMCR- 1999/10/01
CRDT- 1998/11/21 00:00
PHST- 1998/11/21 00:00 [pubmed]
PHST- 1998/11/21 00:01 [medline]
PHST- 1998/11/21 00:00 [entrez]
PHST- 1999/10/01 00:00 [pmc-release]
AID - 421707 [pii]
AID - 10.1023/a:1022542406056 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 1998 Oct;15(9):570-4. doi: 10.1023/a:1022542406056.