PMID- 9824159
OWN - NLM
STAT- MEDLINE
DCOM- 19981210
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 17
IP  - 19
DP  - 1998 Nov 12
TI  - Characterization of the mouse Men1 gene and its expression during development.
PG  - 2485-93
AB  - The gene responsible for multiple endocrine neoplasia type 1 (MEN1), a heritable 
      predisposition to endocrine tumours in man, has recently been identified. Here we 
      have characterized the murine homologue with regard to cDNA sequence, genomic 
      structure, expression pattern and chromosomal localisation. The murine Men1 gene 
      spans approximately 6.7 kb of genomic DNA and is comprised of 10 exons with 
      similar genomic structure to the human locus. It was mapped to the 
      pericentromeric region of mouse chromosome 19, which is conserved with the human 
      11q13 band where MEN1 is located. The predicted protein is 611 amino acids in 
      length and overall is 97% homologous to the human orthologue. The 45 reported 
      MEN1 mutations which alter or delete a single amino acid in human all occur at 
      conserved residues, thereby supporting their functional significance. Two 
      transcripts of approximately 3.2 and 2.8 kb were detected in both embryonal and 
      adult murine tissues, resulting from alternative splicing of intron 1. By RNA in 
      situ hybridization and Northern analysis the spatiotemporal expression pattern of 
      Men1 was determined during mouse development. Men1 gene activity was detected 
      already at gestational day 7. At embryonic day 14 expression was generally high 
      throughout the embryo, while at day 17 the thymus, skeletal muscle, and CNS 
      showed the strongest signal. In selected tissues from postnatal mouse Men1 was 
      detected in all tissues analysed and was expressed at high levels in cerebral 
      cortex, hippocampus, testis, and thymus. In brain the menin protein was detected 
      mainly in nerve cell nuclei, whereas in testis it appeared perinuclear in 
      spermatogonia. These results show that Men1 expression is not confined to organs 
      affected in MEN1, suggesting that Men1 has a significant function in many 
      different cell types including the CNS and testis.
FAU - Stewart, C
AU  - Stewart C
AD  - Queensland Cancer Fund Research Laboratories, Queensland Institute of Medical 
      Research, PO Royal Brisbane Hospital, Herston, Australia.
FAU - Parente, F
AU  - Parente F
FAU - Piehl, F
AU  - Piehl F
FAU - Farnebo, F
AU  - Farnebo F
FAU - Quincey, D
AU  - Quincey D
FAU - Silins, G
AU  - Silins G
FAU - Bergman, L
AU  - Bergman L
FAU - Carle, G F
AU  - Carle GF
FAU - Lemmens, I
AU  - Lemmens I
FAU - Grimmond, S
AU  - Grimmond S
FAU - Xian, C Z
AU  - Xian CZ
FAU - Khodei, S
AU  - Khodei S
FAU - Teh, B T
AU  - Teh BT
FAU - Lagercrantz, J
AU  - Lagercrantz J
FAU - Siggers, P
AU  - Siggers P
FAU - Calender, A
AU  - Calender A
FAU - Van de Vem, V
AU  - Van de Vem V
FAU - Kas, K
AU  - Kas K
FAU - Weber, G
AU  - Weber G
FAU - Hayward, N
AU  - Hayward N
FAU - Gaudray, P
AU  - Gaudray P
FAU - Larsson, C
AU  - Larsson C
LA  - eng
SI  - GENBANK/AF016398
SI  - GENBANK/AF024513
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA, Complementary)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/embryology/metabolism
MH  - Chromosome Mapping
MH  - DNA, Complementary/genetics
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Gene Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice/embryology/*genetics/growth & development
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*biosynthesis
MH  - Nerve Tissue Proteins/biosynthesis/genetics
MH  - Organ Specificity
MH  - *Proto-Oncogene Proteins
MH  - *Proto-Oncogenes
MH  - RNA Splicing
MH  - RNA, Messenger/biosynthesis
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Testis/embryology/metabolism
EDAT- 1998/11/21 00:00
MHDA- 1998/11/21 00:01
CRDT- 1998/11/21 00:00
PHST- 1998/11/21 00:00 [pubmed]
PHST- 1998/11/21 00:01 [medline]
PHST- 1998/11/21 00:00 [entrez]
AID - 10.1038/sj.onc.1202164 [doi]
PST - ppublish
SO  - Oncogene. 1998 Nov 12;17(19):2485-93. doi: 10.1038/sj.onc.1202164.