PMID- 9824475
OWN - NLM
STAT- MEDLINE
DCOM- 19990128
LR  - 20190512
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 95
IP  - 2
DP  - 1998 Oct
TI  - Oral tolerance induced to house dust mite extract in naive and sensitized mice: 
      evaluation of immunoglobulin G anti-immunoglobulin E autoantibodies and IgG-IgE 
      complexes.
PG  - 193-9
AB  - We investigated the effect on specific antibody response of naive and sensitized 
      mice orally administrated with low (0.25 mg) or high (10.0 mg) doses of 
      Dermatophagoides pteronyssinus (Dp) extract. We also examined the effect of oral 
      administration of Dp on the production of autoantibodies to immunoglobulin G 
      (IgG) and immunoglobulin E (IgE). Naive and sensitized mice both showed a marked 
      down-regulation of IgE antibody production, regardless of the dose of Dp. We also 
      detected an inhibitory effect of the total IgE levels and the allergen-specific 
      IgG1, IgG2a and IgG2b antibody response in sensitized mice given the low dose of 
      Dp. In contrast, high doses of Dp stimulated IgG1 antibody production in both 
      naive and sensitized animals. In addition, the oral tolerance induction protocol 
      stimulated anti-F(ab')2gamma and anti-Fcgamma autoantibody production. Evaluation 
      of IgG anti-IgE autoantibodies by a direct enzyme immunoassay (EIA) revealed the 
      presence of these autoantibodies, predominantly of the IgG1 isotype, specifically 
      in those animals fed with the high dose. In contrast, IgG-IgE complexes, 
      determined by EIA using immobilized anti-IgE antibodies, were detected mainly in 
      sera of control animals. The autoantibody anti-IgE specificity was tested against 
      IgE-TNP and IgE-DANSYL murine proteins and revealed different inhibition 
      profiles, suggesting the action of heterogeneous subpopulations of 
      autoantibodies. Taken together, our results show that the oral tolerance protocol 
      with Dp was able to modulate the production of allergen-specific IgE antibodies 
      in both naive and sensitized animals. In addition, we suggest that anti-IgE 
      autoantibodies participate in the modulation of allergic response triggered by 
      oral tolerance protocols.
FAU - Sato, M N
AU  - Sato MN
AD  - Laboratorio de Imunogenetica e Transplante Experimental LIM-56, Faculdade de 
      Medicina da USP (FMUSP), Sao Paulo, Brasil.
FAU - Carvalho, A F
AU  - Carvalho AF
FAU - Silva, A O
AU  - Silva AO
FAU - MacIel, M Jr
AU  - MacIel M Jr
FAU - Fusaro, A E
AU  - Fusaro AE
FAU - Duarte, A J
AU  - Duarte AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (anti-IgE antibodies)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Administration, Oral
MH  - Allergens/*administration & dosage
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/*biosynthesis
MH  - Antibody Formation/drug effects
MH  - Antigen-Antibody Complex/analysis
MH  - Antigens, Dermatophagoides
MH  - Dose-Response Relationship, Immunologic
MH  - Female
MH  - Glycoproteins/*administration & dosage
MH  - Immune Tolerance/*immunology
MH  - Immunoenzyme Techniques
MH  - Immunoglobulin E/*immunology
MH  - Immunoglobulin G/*biosynthesis
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mites
MH  - Passive Cutaneous Anaphylaxis
MH  - Rats
MH  - Rats, Wistar
PMC - PMC1364304
EDAT- 1998/11/21 00:00
MHDA- 1998/11/21 00:01
PMCR- 1999/10/01
CRDT- 1998/11/21 00:00
PHST- 1998/11/21 00:00 [pubmed]
PHST- 1998/11/21 00:01 [medline]
PHST- 1998/11/21 00:00 [entrez]
PHST- 1999/10/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2567.1998.00594.x [doi]
PST - ppublish
SO  - Immunology. 1998 Oct;95(2):193-9. doi: 10.1046/j.1365-2567.1998.00594.x.