PMID- 9827016
OWN - NLM
STAT- MEDLINE
DCOM- 19981208
LR  - 20191102
IS  - 1367-8280 (Print)
IS  - 1367-8280 (Linking)
VI  - 120
IP  - 1
DP  - 1998 Jul
TI  - Comparison of glucokinase activities in the peripheral leukocytes between dogs 
      and cats.
PG  - 53-6
AB  - Activities of hexokinase (HK), glucokinase (GK) and pyruvate kinase (PK), were 
      measured. The expression of GK mRNA was investigated using reverse 
      transcription-polymerase chain reaction (RT-PCR) in leukocytes (WBC) of dogs and 
      cats. No significant differences between dogs and cats were found in 
      concentrations of blood glucose and plasma insulin. Dog WBC showed GK activities 
      and the specific fragment with predicted size of 574 bp containing conserved 
      region including glucose- and ATP-binding domains of GK as determined with 
      RT-PCR. However, in cat WBC, the activities and specific fragment of GK were 
      absent. After fasting, the activities and gene expression of GK decreased greatly 
      in the dog WBC. The cat WBC had significantly higher activities of HK and PK than 
      dog WBC.
FAU - Arai, T
AU  - Arai T
AD  - Division of Veterinary Biochemistry, Nippon Veterinary and Animal Science 
      University, Tokyo, Japan.
FAU - Kawaue, T
AU  - Kawaue T
FAU - Abe, M
AU  - Abe M
FAU - Kuramoto, E
AU  - Kuramoto E
FAU - Kawakami, E
AU  - Kawakami E
FAU - Sako, T
AU  - Sako T
FAU - Washizu, T
AU  - Washizu T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Comp Biochem Physiol C Pharmacol Toxicol Endocrinol
JT  - Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & 
      endocrinology
JID - 9516060
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.2 (Glucokinase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blood Glucose/metabolism
MH  - Cats
MH  - Conserved Sequence
MH  - Cytosol/enzymology
MH  - Dogs
MH  - Female
MH  - Glucokinase/*blood
MH  - Insulin/blood
MH  - Leukocytes/*enzymology
MH  - Male
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Species Specificity
EDAT- 1998/11/25 00:00
MHDA- 1998/11/25 00:01
CRDT- 1998/11/25 00:00
PHST- 1998/11/25 00:00 [pubmed]
PHST- 1998/11/25 00:01 [medline]
PHST- 1998/11/25 00:00 [entrez]
AID - 10.1016/s0742-8413(98)00005-x [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1998 Jul;120(1):53-6. doi: 
      10.1016/s0742-8413(98)00005-x.