PMID- 9829316
OWN - NLM
STAT- MEDLINE
DCOM- 19990210
LR  - 20191027
IS  - 1061-6128 (Print)
IS  - 1061-6128 (Linking)
VI  - 7
IP  - 5
DP  - 1998 Oct
TI  - Fluorescence in situ hybridization for the BCR/ABL rearrangement is dependent on 
      the percentage of nonlymphocytic cells in peripheral blood stem cell harvests.
PG  - 425-30
AB  - Interphase fluorescence in situ hybridization (FISH) for the translocation 
      t(9;22) is widely used for quantifying minimal residual disease (MRD) in PBSC 
      harvests from CML patients. We investigated the influence of cell composition on 
      the percentage of positive FISH signals in 17 BCR/ABL-positive leukapheresis 
      products from 12 CML patients. In these PBSC harvests, a significant correlation 
      between the percentage of nonlymphocytic nucleated cells and BCR/ABL positivity 
      was measured (k=0.81). This correlation was not seen in patients who became 
      BCR/ABL negative after mini-ICE chemotherapy. CD34 enrichment was performed by 
      immunomagnetic separation in 7 patients. There was a statistically significant 
      increase in BCR/ABL positivity after CD34+ selection (p=0.018). This may have 
      been caused by passive depletion of BCR/ABL-negative lymphocytes. Our findings 
      suggest that quantitative results of t(9;22) FISH have to be corrected for cell 
      composition when comparing different stem cell products. CD34+ selection before 
      FISH analysis may be one way to enrich for nonlymphocytic cells and to 
      concentrate on the progenitor compartment.
FAU - Bornhauser, M
AU  - Bornhauser M
AD  - Medizinische Klinik I, Universitatsklinikum Carl Gustav Carus, Dresden, Germany.
FAU - Mohr, B
AU  - Mohr B
FAU - Ehrenlechner, U
AU  - Ehrenlechner U
FAU - Neubauer, A
AU  - Neubauer A
FAU - Ehninger, G
AU  - Ehninger G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Hematother
JT  - Journal of hematotherapy
JID - 9306048
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Blood Cell Count
MH  - *Chromosomes, Human, Pair 22
MH  - *Chromosomes, Human, Pair 9
MH  - Fusion Proteins, bcr-abl/*analysis/genetics
MH  - Hematopoietic Stem Cell Mobilization
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood/*genetics/pathology
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic
EDAT- 1998/11/26 00:00
MHDA- 1998/11/26 00:01
CRDT- 1998/11/26 00:00
PHST- 1998/11/26 00:00 [pubmed]
PHST- 1998/11/26 00:01 [medline]
PHST- 1998/11/26 00:00 [entrez]
AID - 10.1089/scd.1.1998.7.425 [doi]
PST - ppublish
SO  - J Hematother. 1998 Oct;7(5):425-30. doi: 10.1089/scd.1.1998.7.425.