PMID- 9829756
OWN - NLM
STAT- MEDLINE
DCOM- 19990223
LR  - 20121115
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 4
IP  - 11
DP  - 1998 Nov
TI  - Flavopiridol mediates cell cycle arrest and apoptosis in esophageal cancer cells.
PG  - 2885-90
AB  - Esophageal adenocarcinoma (SKGT-2, SKGT-4, and SKGT-5) and epidermoid carcinoma 
      (HCE-4) cells containing variable retinoblastoma (Rb), cyclin D1, p16, and p53 
      expression patterns were exposed to the synthetic flavone, flavopiridol. The IC50 
      was approximately 100-150 nM for each of these cell lines. Exposure of esophageal 
      carcinoma cells to 300 nM flavopiridol induced cell cycle arrest and apoptosis, 
      resulting in a 90% inhibition of proliferation relative to that of nontreated 
      cells after a 5-day exposure to the drug. Western blot analysis revealed 
      diminution of cyclin D1, Rb, and p107 protein levels after flavopiridol exposure. 
      Whereas cell cycle arrest and overall growth inhibition did not correlate in any 
      obvious manner with the genotype of these cell lines, apoptosis seemed to be more 
      pronounced in SKGT-2 and SKGT-4 cells that lack Rb expression. Pretreatment of 
      esophageal cancer cells with 9-cis-retinoic acid did not substantially potentiate 
      flavopiridol activity in these cell lines. Although the precise mechanism of 
      flavopiridol-mediated cytotoxicity has not been fully defined, this drug is an 
      attractive agent for molecular intervention in esophageal cancers and their 
      precursor lesions; further evaluation of flavopiridol in this clinical context is 
      warranted.
FAU - Schrump, D S
AU  - Schrump DS
AD  - Thoracic Oncology Section, Surgery Branch, National Cancer Institute, NIH, 
      Bethesda, Maryland 20892-1502, USA.
FAU - Matthews, W
AU  - Matthews W
FAU - Chen, G A
AU  - Chen GA
FAU - Mixon, A
AU  - Mixon A
FAU - Altorki, N K
AU  - Altorki NK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Flavonoids)
RN  - 0 (Piperidines)
RN  - 45AD6X575G (alvocidib)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - *Apoptosis
MH  - Cell Cycle/*drug effects
MH  - Cell Division/drug effects
MH  - Esophageal Neoplasms/*drug therapy
MH  - Flavonoids/*pharmacology
MH  - Humans
MH  - Piperidines/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1998/11/26 00:00
MHDA- 1998/11/26 00:01
CRDT- 1998/11/26 00:00
PHST- 1998/11/26 00:00 [pubmed]
PHST- 1998/11/26 00:01 [medline]
PHST- 1998/11/26 00:00 [entrez]
PST - ppublish
SO  - Clin Cancer Res. 1998 Nov;4(11):2885-90.