PMID- 9831397
OWN - NLM
STAT- MEDLINE
DCOM- 19981207
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 219
IP  - 1-2
DP  - 1998 Oct 1
TI  - Variability in the sensitivity of nine enzyme-linked immunosorbant assays 
      (ELISAs) in the measurement of human interleukin 6.
PG  - 161-7
AB  - ELISAs employing several combinations of polyclonal and monoclonal anti-human 
      IL-6 antibodies for the detection of interleukin 6 (IL-6) in cell culture 
      supernatants were developed and compared. They were found to have differing 
      sensitivities in the measurement of standard preparations of recombinant human 
      (rh) IL-6 (WHO reference standard) as well as conventional preparations of IL-6 
      produced by stimulated human peripheral blood mononuclear cells. Thus, an ELISA 
      that was optimal for detecting rhIL-6 standard was suboptimal for detecting IL-6 
      in cell culture supernatants. The presence of soluble IL-6 receptor (sIL-6R) or 
      the signal-transducing gp130 molecule or serum did not alter the levels of IL-6 
      detected by these ELISAs. Therefore, the observed variability in sensitivity of 
      the assays may be due to differences in epitope specificity of the various 
      monoclonal antibodies used and the heterogeneity of IL-6 secreted into culture 
      supernatants.
FAU - Krakauer, T
AU  - Krakauer T
AD  - Department of Immunology and Molecular Biology, United States Army Medical 
      Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 
      21702-5011, USA.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antigens, CD)
RN  - 0 (Blood Proteins)
RN  - 0 (Enterotoxins)
RN  - 0 (IL6ST protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Superantigens)
RN  - 133483-10-0 (Cytokine Receptor gp130)
RN  - 39424-53-8 (enterotoxin B, staphylococcal)
SB  - IM
MH  - Antibody Specificity
MH  - Antigens, CD/analysis
MH  - Blood Proteins/analysis
MH  - Cells, Cultured
MH  - Cytokine Receptor gp130
MH  - Enterotoxins/immunology
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Humans
MH  - Interleukin-6/*analysis/immunology
MH  - Leukocytes, Mononuclear
MH  - Membrane Glycoproteins/analysis
MH  - Receptors, Interleukin-6/analysis
MH  - Recombinant Proteins/immunology
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solubility
MH  - Superantigens/immunology
MH  - World Health Organization
EDAT- 1998/11/27 00:00
MHDA- 1998/11/27 00:01
CRDT- 1998/11/27 00:00
PHST- 1998/11/27 00:00 [pubmed]
PHST- 1998/11/27 00:01 [medline]
PHST- 1998/11/27 00:00 [entrez]
AID - S0022-1759(98)00138-0 [pii]
AID - 10.1016/s0022-1759(98)00138-0 [doi]
PST - ppublish
SO  - J Immunol Methods. 1998 Oct 1;219(1-2):161-7. doi: 10.1016/s0022-1759(98)00138-0.