PMID- 9832415
OWN - NLM
STAT- MEDLINE
DCOM- 19981224
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 139
IP  - 12
DP  - 1998 Dec
TI  - Effects of tumor necrosis factor-alpha on glucose metabolism in cultured human 
      muscle cells from nondiabetic and type 2 diabetic subjects.
PG  - 4793-800
AB  - The effects of tumor necrosis factor-alpha (TNF alpha) on glucose uptake and 
      glycogen synthase (GS) activity were studied in human skeletal muscle cell 
      cultures from nondiabetic and type 2 diabetic subjects. In nondiabetic muscle 
      cells, acute (90-min) exposure to TNF alpha (5 ng/ml) stimulated glucose uptake 
      (73 +/- 14% increase) to a greater extent than insulin (37 +/- 4%; P < 0.02). The 
      acute uptake response to TNF alpha in diabetic cells (51 +/- 6% increase) was 
      also greater than that to insulin (31 +/- 3%; P < 0.05). Prolonged (24-h) 
      exposure of nondiabetic muscle cells to TNF alpha resulted in a further 
      stimulation of uptake (152 +/- 31%; P < 0.05), whereas the increase in cells from 
      type 2 diabetics was not significant compared with that in cells receiving acute 
      treatment. After TNF alpha treatment, the level of glucose transporter-1 protein 
      was elevated in nondiabetic (4.6-fold increase) and type 2 (1.7-fold) cells. 
      Acute TNF alpha treatment had no effect on the fractional velocity of GS in 
      either nondiabetic or type 2 cells. Prolonged exposure reduced the GS fractional 
      velocity in both nondiabetic and diabetic cells. In summary, both acute and 
      prolonged treatment with TNF alpha up-regulate glucose uptake activity in 
      cultured human muscle cells, but reduce GS activity. Increased skeletal muscle 
      glucose uptake in conditions of TNF alpha excess may serve as a compensatory 
      mechanism in the insulin resistance of type 2 diabetes.
FAU - Ciaraldi, T P
AU  - Ciaraldi TP
AD  - Veterans Affairs Medical Center and Department of Medicine, University of 
      California-San Diego, La Jolla, California 92093, USA. tciaraldi@ucsd.edu
FAU - Carter, L
AU  - Carter L
FAU - Mudaliar, S
AU  - Mudaliar S
FAU - Kern, P A
AU  - Kern PA
FAU - Henry, R R
AU  - Henry RR
LA  - eng
GR  - DK-39176/DK/NIDDK NIH HHS/United States
GR  - MO1-RR-00827/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.4.1.11 (Glycogen Synthase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Diabetes Mellitus, Type 2/*metabolism/pathology
MH  - Glucose/*metabolism
MH  - Glycogen/biosynthesis
MH  - Glycogen Synthase/metabolism
MH  - Humans
MH  - Middle Aged
MH  - Monosaccharide Transport Proteins/metabolism
MH  - Muscle, Skeletal/*drug effects/*metabolism/pathology
MH  - Reference Values
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1998/12/01 00:00
MHDA- 1998/12/01 00:01
CRDT- 1998/12/01 00:00
PHST- 1998/12/01 00:00 [pubmed]
PHST- 1998/12/01 00:01 [medline]
PHST- 1998/12/01 00:00 [entrez]
AID - 10.1210/endo.139.12.6368 [doi]
PST - ppublish
SO  - Endocrinology. 1998 Dec;139(12):4793-800. doi: 10.1210/endo.139.12.6368.