PMID- 9832646
OWN - NLM
STAT- MEDLINE
DCOM- 19990210
LR  - 20220318
IS  - 1660-8151 (Print)
IS  - 1660-8151 (Linking)
VI  - 80
IP  - 4
DP  - 1998 Dec
TI  - Renal ischemia-reperfusion injury: contribution of nitric oxide and renal blood 
      flow.
PG  - 458-67
AB  - The contributions of nitric oxide (NO) and renal blood flow (RBF) were examined 
      in ischemia-reperfusion injury in the rat kidney. The function of both kidneys 
      was assessed by glomerular filtration rate (GFR), and fractional excretion of 
      sodium (FENa), calculated before, during unilateral renal artery clamping (45 
      min), and following reperfusion (90 min). RBF was measured in the same model by 
      ultrasonic flowmetry. Intrarenal NO levels were modulated by administration of 
      S-nitroso-N-acetylpenicillamine (SNAP), L-arginine, acetylcholine, and the NO 
      synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME). SNAP increased GFR 
      from 0.20 +/- 0.04 ml/min in control ischemic kidney to 0.38 +/- 0.06 ml/min and 
      reduced FENa from 19.3 +/- 3.4 to 9.5 +/- 1.8%. Similar results were observed 
      when L-arginine was administered. Acetylcholine had no effect on GFR or FENa. RBF 
      was fully restored within 60 min following reperfusion, with no change in the 
      rate of recovery by L-arginine. L-NAME aggravated the ischemia-reperfusion 
      injury, preventing full restoration of RBF, further reducing GFR and worsening 
      FENa. In conclusion, ischemia-reperfusion injury ends in low intrarenal levels of 
      NO. We propose that this low NO level results from damage to the endothelial 
      receptor signal transduction process and is not due to impaired NO synthase 
      activity or to changes in RBF.
FAU - Mashiach, E
AU  - Mashiach E
AD  - Eliachar Research Laboratory, Western Galilee Hospital, Nahariya, Israel. 
      SHSELA@naharia.health.gov.il
FAU - Sela, S
AU  - Sela S
FAU - Winaver, J
AU  - Winaver J
FAU - Shasha, S M
AU  - Shasha SM
FAU - Kristal, B
AU  - Kristal B
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Nephron
JT  - Nephron
JID - 0331777
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (S-nitro-N-acetylpenicillamine)
RN  - 0 (Vasodilator Agents)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 94ZLA3W45F (Arginine)
RN  - 9NEZ333N27 (Sodium)
RN  - GNN1DV99GX (Penicillamine)
RN  - N9YNS0M02X (Acetylcholine)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Arginine/pharmacology
MH  - Blood Pressure/drug effects/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Glomerular Filtration Rate/drug effects
MH  - Ischemia/metabolism/*physiopathology
MH  - Kidney/*blood supply/drug effects/metabolism
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Nitric Oxide/*physiology
MH  - Penicillamine/analogs & derivatives/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Renal Circulation/drug effects/*physiology
MH  - Reperfusion Injury/metabolism/*physiopathology
MH  - Sodium/urine
MH  - Vasodilator Agents/pharmacology
EDAT- 1998/12/02 03:02
MHDA- 2000/08/16 11:00
CRDT- 1998/12/02 03:02
PHST- 1998/12/02 03:02 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1998/12/02 03:02 [entrez]
AID - nef80458 [pii]
AID - 10.1159/000045220 [doi]
PST - ppublish
SO  - Nephron. 1998 Dec;80(4):458-67. doi: 10.1159/000045220.