PMID- 9833769
OWN - NLM
STAT- MEDLINE
DCOM- 19981204
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 78
IP  - 6
DP  - 1998 Dec 9
TI  - Hepatocyte growth factor/scatter factor induces not only scattering but also 
      cohort migration of human colorectal-adenocarcinoma cells.
PG  - 750-9
AB  - We presented earlier a 2-dimensional cell-motility assay using a highly 
      metastatic variant (L-10) of human rectal-adenocarcinoma cell line RCM-1 as a 
      motility model of tumor cells of epithelial origin. In this model, L-10 cells 
      moved as coherent cell sheets when stimulated with 
      12-O-tetradecanoylphorbol-13-acetate (TPA), and we called this type of movement 
      "cohort migration". Electron- and immunoelectron-microscope study of the 
      migrating cell sheets demonstrated localized release from cell-cell adhesion only 
      at the lower portion of the cells with loss of E-cadherin immunoreactivity, and 
      this change was associated with increased tyrosine phosphorylation of the 
      E-cadherin-catenin complex, including beta-catenin. In the present study, to 
      obtain evidence to support the relevance of our model to carcinoma-cell movement 
      in vivo, we sought a naturally occurring motogenic factor(s) able to induce this 
      cohort migration. Among the factors examined, hepatocyte growth factor/scatter 
      factor (HGF/SF) clearly induced cohort migration of L-10 cells. Additionally, not 
      only L-10 but several other human colorectal-carcinoma cell lines showed this 
      type of migration in response to HGF/SF, while yet others showed scattering-type 
      motility. In this HGF/SF-induced migration, localized release from cell-cell 
      adhesion was induced only at the lower portion of the cells, allowing them to 
      extend leading lamellae, whereas close cell-cell contacts remained at the upper 
      portion of the cells, as seen in TPA-induced cohort migration. Scattering-type 
      cell lines tended to express more c-Met (receptor for HGF/SF) mRNA than the cell 
      lines that showed cohort-type migration. LoVo, one of the scattering-type cell 
      lines, expressed more c-Met protein and less E-cadherin than L-10, which showed 
      cohort-type migration. HGF/SF treatment of LoVo reduced the amount of 
      alpha-catenin complexed with E-cadherin more markedly than in L-10, but in both 
      cell lines this reduction was not accompanied by increased tyrosine 
      phosphorylation of beta-catenin, suggesting the presence of a mechanism other 
      than phosphorylation for release from cell-cell adhesion during cell motility.
FAU - Nabeshima, K
AU  - Nabeshima K
AD  - Department of Pathology, Miyazaki Medical College, Kiyotake, Japan.
FAU - Shimao, Y
AU  - Shimao Y
FAU - Inoue, T
AU  - Inoue T
FAU - Itoh, H
AU  - Itoh H
FAU - Kataoka, H
AU  - Kataoka H
FAU - Koono, M
AU  - Koono M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (CTNNA1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (alpha Catenin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Adenocarcinoma/*pathology
MH  - Cadherins/metabolism
MH  - Cell Adhesion/drug effects
MH  - Cell Division/drug effects
MH  - Cell Movement/drug effects
MH  - Colorectal Neoplasms/*pathology
MH  - Cytoskeletal Proteins/metabolism
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Phosphorylation
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Cells, Cultured
MH  - alpha Catenin
EDAT- 1998/12/02 03:03
MHDA- 2000/06/20 09:00
CRDT- 1998/12/02 03:03
PHST- 1998/12/02 03:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/12/02 03:03 [entrez]
AID - 10.1002/(SICI)1097-0215(19981209)78:6<750::AID-IJC13>3.0.CO;2-# [pii]
AID - 10.1002/(sici)1097-0215(19981209)78:6<750::aid-ijc13>3.0.co;2-# [doi]
PST - ppublish
SO  - Int J Cancer. 1998 Dec 9;78(6):750-9. doi: 
      10.1002/(sici)1097-0215(19981209)78:6<750::aid-ijc13>3.0.co;2-#.