PMID- 9835285
OWN - NLM
STAT- MEDLINE
DCOM- 19990222
LR  - 20061115
IS  - 0145-6008 (Print)
IS  - 0145-6008 (Linking)
VI  - 22
IP  - 8
DP  - 1998 Nov
TI  - Chemokine and cell adhesion molecule mRNA expression and neutrophil infiltration 
      in lipopolysaccharide-induced hepatitis in ethanol-fed rats.
PG  - 1713-8
AB  - Neutrophil infiltration is a feature of alcoholic hepatitis (AH), and although 
      the mechanism by which this occurs is unclear, it may involve a chemotactic 
      gradient. We used lipopolysaccharide (LPS) to induce, in ethanol-fed rats, liver 
      damage similar to that seen in AH. To our knowledge, this study is the first to 
      examine the effect of ethanol on LPS-stimulated chemokine mRNA expression in this 
      model. Hepatic cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-2, 
      monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein 
      (MIP)-1beta, MIP-2, and eotaxin mRNA levels were elevated 1 to 3 hr post-LPS in 
      both groups. Maximal expression of MIP-2 and MCP-1 mRNA was higher in ethanol-fed 
      rats 1 hr post-LPS, whereas CINC-2 mRNA expression was elevated above controls at 
      12 to 24 hr. Hepatic intercellular adhesion molecule-1 and vascular cell adhesion 
      molecule-1 mRNA levels were elevated in both groups at 1 hr, whereas L-selectin 
      expression in ethanol-fed rats was elevated above controls at 12 to 24 hr. 
      Hepatic neutrophil infiltration was highest during maximal hepatocyte necrosis. 
      These data suggest that cell adhesion molecules, in conjunction with elevated 
      cytokines and the subsequently induced chemokines, may assist in the formation of 
      a chemotactic gradient within the liver, causing the neutrophil infiltration seen 
      both in this model and possibly in AH.
FAU - Pennington, H L
AU  - Pennington HL
AD  - Department of Biochemistry, The University of Queensland, St. Lucia, Australia.
FAU - Wilce, P A
AU  - Wilce PA
FAU - Worrall, S
AU  - Worrall S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Alcohol Clin Exp Res
JT  - Alcoholism, clinical and experimental research
JID - 7707242
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules/*genetics
MH  - Chemokines/*genetics
MH  - Gene Expression/drug effects
MH  - Hepatitis, Alcoholic/*genetics/immunology
MH  - Lipopolysaccharides/*immunology
MH  - Liver Cirrhosis, Alcoholic/genetics/immunology
MH  - Male
MH  - Neutrophils/drug effects/immunology
MH  - RNA, Messenger/*genetics
MH  - Rats
MH  - Rats, Wistar
EDAT- 1998/12/03 00:00
MHDA- 1998/12/03 00:01
CRDT- 1998/12/03 00:00
PHST- 1998/12/03 00:00 [pubmed]
PHST- 1998/12/03 00:01 [medline]
PHST- 1998/12/03 00:00 [entrez]
AID - 00000374-199811000-00014 [pii]
PST - ppublish
SO  - Alcohol Clin Exp Res. 1998 Nov;22(8):1713-8.