PMID- 9838090
OWN - NLM
STAT- MEDLINE
DCOM- 19990316
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 814
IP  - 1-2
DP  - 1998 Dec 14
TI  - Resistance of the hippocampus in the lactating rat to N-methyl-D-aspartate 
      (NMDA)-mediated excitation is not due to a nonfunctional receptor system.
PG  - 157-63
AB  - The lactating rat has been shown to lack a behavioral response and immediate 
      early gene expression (cFos) in the hippocampus (Hipc) following intravenous or 
      intracerebroventricular administration of an N-methyl-d-aspartate (NMDA) receptor 
      agonist. The purpose of this study is to determine whether neurons in the Hipc 
      have an intact postsynaptic NMDA receptor system. The presence of NMDA receptor 
      protein was determined by Western blot analysis for the NR1, NR2A, and NR2B 
      subunits. The presence of functional NMDA receptors in the Hipc was determined by 
      behavioral responses and the expression of cFos immunoreactivity (-ir) in 
      response to microinjection of an NMDA receptor agonist into the hilus of the 
      dentate gyrus. No difference in NR1 and NR2A subunit protein in the Hipc was 
      detected between the lactating and nonlactating rats. However, there was a 26% 
      decrease in NR2B subunit protein in this region in the lactating rat. Lactating 
      rats receiving NMA injections displayed hyperactive behavior, similar to that 
      observed in the nonlactating animals receiving the same treatment. The lactating 
      rat and the nonlactating rat also displayed equivalent bilateral cFos-ir in the 
      dentate gyrus (DG), CA1 and CA3 regions of the Hipc in response to unilateral NMA 
      injections into the Hipc. These data indicate that the lactating rat has an 
      intact postsynaptic NMDA receptor system. Thus, Hipc refractoriness to peripheral 
      and third ventricular injections of an NMDA receptor agonist may reflect 
      inhibition of presynaptic input and glutamate release.
CI  - Copyright 1998 Elsevier Science B.V.
FAU - Grove, K L
AU  - Grove KL
AD  - Oregon Regional Primate Research Center, Division of Neuroscience, 505 NW 185th, 
      Beaverton, OR 97006, USA.grovek@ohsu.edu
FAU - Smith, M S
AU  - Smith MS
LA  - eng
GR  - HD14643/HD/NICHD NIH HHS/United States
GR  - RR00163/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Blotting, Western
MH  - Female
MH  - Genes, fos
MH  - Hippocampus/*physiology
MH  - Injections, Intraventricular
MH  - Lactation/*physiology
MH  - N-Methylaspartate/*physiology
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/*physiology
EDAT- 1998/12/05 00:00
MHDA- 1998/12/05 00:01
CRDT- 1998/12/05 00:00
PHST- 1998/12/05 00:00 [pubmed]
PHST- 1998/12/05 00:01 [medline]
PHST- 1998/12/05 00:00 [entrez]
AID - S0006-8993(98)01069-5 [pii]
AID - 10.1016/s0006-8993(98)01069-5 [doi]
PST - ppublish
SO  - Brain Res. 1998 Dec 14;814(1-2):157-63. doi: 10.1016/s0006-8993(98)01069-5.