PMID- 9840345
OWN - NLM
STAT- MEDLINE
DCOM- 19990129
LR  - 20190915
IS  - 0300-5623 (Print)
IS  - 0300-5623 (Linking)
VI  - 26
IP  - 5
DP  - 1998
TI  - Role of superoxide anion on the proliferation and c-Ha-ras or p53 expression in 
      prostate cancer cell line PC3.
PG  - 349-53
AB  - The purpose of this study was to investigate the role of superoxide anion (02-*) 
      in the regulation of p53 or c-Ha-ras expression and proliferation in the prostate 
      cancer cell line PC3. Cell proliferation was tested by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in the 
      presence of O2-*, basic fibroblast growth factor (bFGF) or their combination. p53 
      or C-Ha-ras expression in the cells treated with O2-* was assayed by fluorescence 
      in situ hybridization (FISH). The proliferation was significantly inhibited by 
      O2-* in a concentration-dependent manner ranging from 9 to 36 micromol/l 
      nicotinamide adenine dinucleotid (NADH) combined with 2-8 micromol/l 
      N-methylphenazonium methyl sulfate (PMS). Enhancement of proliferation by 2 ng/ml 
      bFGF was significantly inhibited by O2-*. Although O2-* was not able to alter 
      c-Ha-ras gene expression, O2-* at the concentrations of 18 micromol/l NADH and 4 
      micromol/l PMS upregulated the expression of p53. O2-* may modulate proliferation 
      and gene expression in PC3 cells.
FAU - Wang, Z
AU  - Wang Z
AD  - Department of Biology, Lanzhou University, P.R. China.
FAU - Zheng, R
AU  - Zheng R
FAU - Fu, S
AU  - Fu S
FAU - Chen, Y
AU  - Chen Y
FAU - Duan, G
AU  - Duan G
FAU - Qin, D
AU  - Qin D
FAU - Liu, G
AU  - Liu G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Urol Res
JT  - Urological research
JID - 0364311
RN  - 0 (Anions)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 11062-77-4 (Superoxides)
RN  - EC 3.6.5.2 (Oncogene Protein p21(ras))
SB  - IM
MH  - Anions/metabolism
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Gene Expression Regulation, Neoplastic/drug effects/*physiology
MH  - Humans
MH  - Male
MH  - Oncogene Protein p21(ras)/*genetics
MH  - *Prostatic Neoplasms
MH  - Superoxides/metabolism/*pharmacology
MH  - Tumor Cells, Cultured/drug effects/physiology
MH  - Tumor Suppressor Protein p53/*genetics
EDAT- 1998/12/05 00:00
MHDA- 1998/12/05 00:01
CRDT- 1998/12/05 00:00
PHST- 1998/12/05 00:00 [pubmed]
PHST- 1998/12/05 00:01 [medline]
PHST- 1998/12/05 00:00 [entrez]
AID - 10.1007/s002400050068 [doi]
PST - ppublish
SO  - Urol Res. 1998;26(5):349-53. doi: 10.1007/s002400050068.