PMID- 9840941
OWN - NLM
STAT- MEDLINE
DCOM- 19981222
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 17
IP  - 21
DP  - 1998 Nov 26
TI  - Chimeric amplicons containing the c-myc gene in HL60 cells.
PG  - 2771-7
AB  - The major amplicon present in HL60 cells is chimeric in nature being composed of 
      70 kb of DNA sequence derived from the MYC locus linked to 80 kb of novel DNA 
      sequence derived from a non contiguous region located telomeric to the c-myc gene 
      at 8q24 (Feo et al., 1996). Here we show by fluorescence in situ hybridization 
      (FISH) that these coamplified sequences, MCR (Myc Coamplified Region), are 
      derived from a locus located 3-4 Mb telomeric to the c-myc gene in the q24.2-24.3 
      region of chromosome 8. Genomic cloning and Southern blot analysis indicate the 
      arrangement of chimeric amplicons are in tandem arrays. Analysis of the DNA 
      sequences at the juncture of the MYC locus and the MCR suggest that these non 
      syntenic regions were joined by nonhomologous recombination events. Visualization 
      of the organization of the amplified DNA by fiber-FISH analysis illustrates we 
      have cloned the complete amplicon. This is the first complete mammalian amplicon 
      to be cloned and have its structure visualized. In addition to the major class of 
      tandemly repeated amplicons, a second class of amplicons was detected by 
      fiber-FISH in which the extent of the MCR component is about twice the size of 
      the MCR component in the major amplicon. These longer amplicons most likely 
      contain inverted repeats of MCR and MYC region sequences. Whether the amplicons 
      contain mixtures of these two types of structures or separate amplicons only 
      contain one type of structure has not yet been resolved. Properties of the MCR 
      sequences responsible for retention in the chimeric HL60 amplicons upon long term 
      passage are discussed.
FAU - Mangano, R
AU  - Mangano R
AD  - Dipartimento di Biologia Cellulare e dello Sviluppo, Universita di Palermo, 
      Italy.
FAU - Piddini, E
AU  - Piddini E
FAU - Carramusa, L
AU  - Carramusa L
FAU - Duhig, T
AU  - Duhig T
FAU - Feo, S
AU  - Feo S
FAU - Fried, M
AU  - Fried M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Oncogene Proteins, Fusion)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Southern
MH  - *Chromosome Aberrations
MH  - *Chromosome Fragility
MH  - Chromosomes, Human, Pair 8/*genetics/ultrastructure
MH  - Cloning, Molecular
MH  - *Gene Amplification
MH  - *Genes, myc
MH  - HL-60 Cells/*chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Promyelocytic, Acute/*genetics/pathology
MH  - Molecular Sequence Data
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Recombination, Genetic
MH  - Sequence Analysis, DNA
EDAT- 1998/12/05 00:00
MHDA- 1998/12/05 00:01
CRDT- 1998/12/05 00:00
PHST- 1998/12/05 00:00 [pubmed]
PHST- 1998/12/05 00:01 [medline]
PHST- 1998/12/05 00:00 [entrez]
AID - 10.1038/sj.onc.1202434 [doi]
PST - ppublish
SO  - Oncogene. 1998 Nov 26;17(21):2771-7. doi: 10.1038/sj.onc.1202434.