PMID- 9843777
OWN - NLM
STAT- MEDLINE
DCOM- 19990203
LR  - 20190221
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 275
IP  - 6
DP  - 1998 Dec
TI  - Zonal differences in effects of HGF/SF and EGF on DNA synthesis in hepatocytes 
      under fed or starved conditions.
PG  - G1394-401
LID - 10.1152/ajpgi.1998.275.6.G1394 [doi]
AB  - Zonal differences of DNA synthesis in hepatocytes induced by hepatocyte growth 
      factor and/or scatter factor (HGF/SF) and epidermal growth factor (EGF) were 
      investigated using male Wistar rats under fed or starved conditions. Overall, DNA 
      synthesis was greater in fed rats than in starved rats. The predominance of EGF 
      in periportal hepatocytes (PPH) on zonal DNA synthesis was reversed by starved 
      conditions, but the predominance of HGF/SF on zonal DNA synthesis in perivenous 
      hepatocytes (PVH) was not influenced by nutritional conditions. 125I-labeled EGF 
      and 125I-labeled HGF/SF-receptor binding studies revealed no significant 
      difference between PPH and PVH in starved or fed rats. To investigate the 
      mechanism of the signal transduction pathway, we used genistein, an inhibitor of 
      tyrosine kinase. Genistein had different effects on zonal difference in EGF and 
      HGF/SF. In EGF, 1 microgram/ml genistein abolished zonal differences, but in 
      HGF/SF 1 microgram/ml genistein did not abolish zonal differences. These data 
      suggest that, in contrast to HGF/SF, zonal difference of DNA synthesis by EGF was 
      dependent on nutritional conditions and DNA synthesis induced by HGF/SF and EGF 
      might be related to tyrosine kinase, but the influence of tyrosine kinase on DNA 
      synthesis was different between HGF/SF and EGF.
FAU - Imai, K
AU  - Imai K
AD  - Departments of Anesthesiology and Internal Medicine IV, University of Tokyo 
      School of Medicine, Bunkyo-ku, Tokyo 112, Japan.
FAU - Mine, T
AU  - Mine T
FAU - Tagami, M
AU  - Tagami M
FAU - Hanaoka, K
AU  - Hanaoka K
FAU - Fujita, T
AU  - Fujita T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Growth Inhibitors)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-49-2 (DNA)
RN  - DH2M523P0H (Genistein)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - DNA/*biosynthesis
MH  - Eating/physiology
MH  - Epidermal Growth Factor/metabolism/*pharmacology
MH  - Fasting/physiology
MH  - Genistein/pharmacology
MH  - Growth Inhibitors/pharmacology
MH  - Hepatocyte Growth Factor/metabolism/*pharmacology
MH  - Liver/cytology/*drug effects/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
MH  - Tissue Distribution
EDAT- 1998/12/09 00:00
MHDA- 1998/12/09 00:01
CRDT- 1998/12/09 00:00
PHST- 1998/12/09 00:00 [pubmed]
PHST- 1998/12/09 00:01 [medline]
PHST- 1998/12/09 00:00 [entrez]
AID - 10.1152/ajpgi.1998.275.6.G1394 [doi]
PST - ppublish
SO  - Am J Physiol. 1998 Dec;275(6):G1394-401. doi: 10.1152/ajpgi.1998.275.6.G1394.