PMID- 9844130
OWN - NLM
STAT- MEDLINE
DCOM- 19990211
LR  - 20061115
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 54
IP  - 5
DP  - 1998 Nov
TI  - Cultured human glomerular mesangial cells express the C5a receptor.
PG  - 1542-9
AB  - BACKGROUND: While the association of complement activation and glomerulonephritis 
      has been recognized for decades, the pathogenic mechanisms of complement-mediated 
      glomerular damage are incompletely understood. Expression of the C5a receptor in 
      the kidney suggests that C5a could play a direct role in initiating or promoting 
      glomerulonephritis. METHODS: Expression of the C5a receptor by cultured human 
      glomerular mesangial cells (HGMC) was examined by immunofluorescence, by FACS 
      analysis and by reverse transcriptase-polymerase chain reaction (RT-PCR). 
      Potential mitogenic effects were examined by analysis of neutral red dye uptake 
      after treatment with recombinant C5a (rC5a). The production of cytokines 
      [interleukin-1 (IL-1), interleukin-8 (IL-8), and monocyte chemoattractant 
      protein-1 (MCP-1)] and growth factors [transforming growth factor-beta (TGF-beta) 
      and platelet-derived growth factor (PDGF-AB)] by mesangial cells stimulated with 
      rC5a was examined by ELISA of cell culture supernatants. RESULTS: Expression of 
      the C5a receptor by the cultured HGMC was demonstrated by both immunofluorescence 
      and FACS. The presence of mRNA encoding the receptor was confirmed by RT-PCR. 
      Treatment of HGMC in vitro with rC5a resulted in mild cellular proliferation. No 
      IL-1 was detected despite stimulation with up to 100 nM rC5a. Concentrations of 
      IL-8 and TGF-beta did not increase beyond basal levels in control samples at any 
      level of stimulation. Mean MCP-1 concentrations and PDGF-AB concentrations 
      increased by 40% and 70% above control values 48 hours post-stimulation (P = 0.01 
      and P = 0.003, respectively). CONCLUSIONS: These data indicate that the C5a 
      receptor is expressed on HGMC in vitro, and may play a role in mediating 
      glomerular injury by promoting cellular proliferation and the production of 
      cytokines and growth factors.
FAU - Braun, M
AU  - Braun M
AD  - Division of Nephrology, Children's Hospital Research Foundation, Cincinnati, 
      Ohio, USA.
FAU - Davis, A E 3rd
AU  - Davis AE 3rd
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Antigens, CD)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Receptor, Anaphylatoxin C5a)
RN  - 0 (Receptors, Complement)
RN  - 80295-54-1 (Complement C5a)
SB  - IM
MH  - Antigens, CD/*analysis/genetics/physiology
MH  - Cell Division
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Complement C5a/physiology
MH  - Fluorescent Antibody Technique
MH  - Glomerular Mesangium/*chemistry/cytology/metabolism
MH  - Humans
MH  - Platelet-Derived Growth Factor/biosynthesis
MH  - Receptor, Anaphylatoxin C5a
MH  - Receptors, Complement/*analysis/genetics/physiology
EDAT- 1998/12/09 03:03
MHDA- 2001/03/28 10:01
CRDT- 1998/12/09 03:03
PHST- 1998/12/09 03:03 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1998/12/09 03:03 [entrez]
AID - S0085-2538(15)30784-5 [pii]
AID - 10.1046/j.1523-1755.1998.00155.x [doi]
PST - ppublish
SO  - Kidney Int. 1998 Nov;54(5):1542-9. doi: 10.1046/j.1523-1755.1998.00155.x.