PMID- 9844608
OWN - NLM
STAT- MEDLINE
DCOM- 19981210
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 107
IP  - 2
DP  - 1998 Dec
TI  - Concurrent cytogenetic, interphase fluorescence in situ hybridization and DNA 
      flow cytometric analyses of a carcinoma ex-pleomorphic adenoma of parotid gland.
PG  - 132-6
AB  - We report the cytogenetic, fluorescence in situ hybridization (FISH), and DNA 
      ploidy analyses of a high grade carcinoma ex-pleomorphic adenoma of the 
      submandibular gland. Our overall combined analyses showed a marked DNA aneuploidy 
      and numerical abnormalities involving all chromosomes. Cytogenetic analysis 
      revealed a near tetraploid modal chromosomal number with tetraploid loss of 
      chromosomes Y, 1, 6, 9, 11, 14, 15, 17, and 19-21 and hypertetraploid gain of 
      chromosomes 7, 8, and 22. The structural abnormalities included 
      der(1;14)(q10;q10), del(6)(q15q34), +del(6)(q15q34), +der(8) 
      t(1;8)(q12;q12.2),der(9;19)(q10;q10),add(14)(p11.2),i(20)(q10),der(21) 
      t(8;21)(q11.2;q22.3),+der(21)t(8;21) (q11.2;q22.3). Interphase FISH of the 
      primary and short-term cultured cells using directly labeled pericentromeric 
      probes for chromosomes 6-12, 17, 18, and Y resulted in alterations corresponding 
      to the cytogenetic findings. DNA ploidy analysis of both the primary and cultured 
      tumor cells showed a hyperdiploid stemline with DNA indices of 2.6. The results 
      indicate that: (1) marked numerical, structural chromosomal, and DNA content 
      abnormalities are present in this tumor; and (2) alteration at 8q and 6q regions, 
      together with previous results, suggest an association between these events and 
      the development and/or progression of this tumor.
FAU - el-Naggar, A K
AU  - el-Naggar AK
AD  - Department of Pathology, University of Texas, M.D. Anderson Cancer Center, 
      Houston, TX 77030, USA.
FAU - Lovell, M
AU  - Lovell M
FAU - Callender, D L
AU  - Callender DL
FAU - Ordonez, N G
AU  - Ordonez NG
FAU - Killary, A M
AU  - Killary AM
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adenocarcinoma/*genetics/pathology
MH  - Adenoma, Pleomorphic/*genetics/pathology
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Parotid Neoplasms/*genetics/pathology
RF  - 37
EDAT- 1998/12/09 00:00
MHDA- 1998/12/09 00:01
CRDT- 1998/12/09 00:00
PHST- 1998/12/09 00:00 [pubmed]
PHST- 1998/12/09 00:01 [medline]
PHST- 1998/12/09 00:00 [entrez]
AID - S0165460898001009 [pii]
AID - 10.1016/s0165-4608(98)00100-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Dec;107(2):132-6. doi: 
      10.1016/s0165-4608(98)00100-9.