PMID- 9846695
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190512
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 10
IP  - 11
DP  - 1998 Nov
TI  - Cell lines transfected with the TAP inhibitor ICP47 allow testing peptide binding 
      to a variety of HLA class I molecules.
PG  - 1665-72
AB  - The immediate early protein ICP47 of the Herpes simplex virus is known to block 
      the human transporter associated with antigen processing (TAP), thereby creating 
      a TAP-deficient phenotype in any human cell transfected with the corresponding 
      cDNA. Exploiting this inhibitory activity, we constructed a selection of human 
      cell lines each co-expressing one of the cDNAs of human leukocyte antigen (HLA) 
      class I alleles HLA-A*1101, A24, A*3101, A*6601, B8 and B*1516, and the cDNA 
      encoding the ICP47 molecule. The cell lines generated showed diminished HLA class 
      I surface expression and the inhibition of the TAP function was confirmed in 
      peptide translocation assays. The addition of specific exogenous peptide ligands 
      restored the expression of the corresponding HLA class I molecules. Thus, the 
      ICP47 transfectants provide us with a tool to closely examine peptide-HLA class I 
      interactions, to confirm HLA class I ligand motifs and to test peptides predicted 
      to bind.
FAU - Gatfield, J
AU  - Gatfield J
AD  - Department of Immunology, Institute for Cell Biology, Eberhard-Karls University 
      Tubingen, Germany.
FAU - Lammert, E
AU  - Lammert E
FAU - Nickolaus, P
AU  - Nickolaus P
FAU - Munz, C
AU  - Munz C
FAU - Rothenfusser, S
AU  - Rothenfusser S
FAU - Fisch, P
AU  - Fisch P
FAU - Stevanovic, S
AU  - Stevanovic S
FAU - Schild, H
AU  - Schild H
FAU - Rammensee, H G
AU  - Rammensee HG
FAU - Arnold, D
AU  - Arnold D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (ICP47 protein, Herpes simplex virus)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Ligands)
RN  - 0 (Oligopeptides)
RN  - 0 (TAP1 protein, human)
RN  - 0 (Tap1 protein, mouse)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters/*antagonists & inhibitors
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Female
MH  - Flow Cytometry
MH  - HLA-A Antigens/metabolism
MH  - HLA-B Antigens/metabolism
MH  - Herpesvirus 2, Human
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Humans
MH  - Immediate-Early Proteins/genetics/*metabolism
MH  - Ligands
MH  - Mice
MH  - Oligopeptides/*metabolism
MH  - Sequence Alignment
MH  - Transfection
MH  - *Viral Proteins
EDAT- 1998/12/10 00:00
MHDA- 1998/12/10 00:01
CRDT- 1998/12/10 00:00
PHST- 1998/12/10 00:00 [pubmed]
PHST- 1998/12/10 00:01 [medline]
PHST- 1998/12/10 00:00 [entrez]
AID - 10.1093/intimm/10.11.1665 [doi]
PST - ppublish
SO  - Int Immunol. 1998 Nov;10(11):1665-72. doi: 10.1093/intimm/10.11.1665.