PMID- 9847336
OWN - NLM
STAT- MEDLINE
DCOM- 19990128
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 73
IP  - 1
DP  - 1999 Jan
TI  - Trafficking to the plasma membrane of the seven-transmembrane protein encoded by 
      human herpesvirus 6 U51 gene involves a cell-specific function present in T 
      lymphocytes.
PG  - 325-33
AB  - The sequence of human herpesvirus 6 (HHV-6) U51 open reading frame predicts a 
      protein of 301 amino acid residues with seven transmembrane domains. To identify 
      and characterize U51, we derived antipeptide polyclonal antibodies and developed 
      a transient expression assay. We ascertained that U51 was synthesized in cord 
      blood mononuclear cells infected with either variant A- or variant B-HHV-6 and 
      was transported to the surface of productively infected cells. When synthesized 
      in transient expression systems, U51 intracellular trafficking was regulated in a 
      cell-type-dependent fashion. In human monolayer HEK-293 and 143tk- cells, U51 
      accumulated predominantly in the endoplasmic reticulum and failed to be 
      transported to the cell surface. In contrast, in T-lymphocytic cell lines J-Jhan, 
      Molt-3, and Jurkat, U51 was successfully transported to the plasma membrane. We 
      infer that transport of U51 to the cell surface requires a cell-specific function 
      present in activated T lymphocytes and T-cell lines.
FAU - Menotti, L
AU  - Menotti L
AD  - Section on Microbiology and Virology, Department of Experimental Pathology, 
      University of Bologna, Bologna, Italy.
FAU - Mirandola, P
AU  - Mirandola P
FAU - Locati, M
AU  - Locati M
FAU - Campadelli-Fiume, G
AU  - Campadelli-Fiume G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Messenger)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Cell Membrane/virology
MH  - Chlorocebus aethiops
MH  - Herpesvirus 6, Human/*physiology
MH  - Humans
MH  - Open Reading Frames
MH  - RNA, Messenger/analysis
MH  - T-Lymphocytes/*virology
MH  - Transfection
MH  - Vero Cells
MH  - Viral Envelope Proteins/genetics/*metabolism
PMC - PMC103837
EDAT- 1998/12/16 00:00
MHDA- 1998/12/16 00:01
PMCR- 1999/01/01
CRDT- 1998/12/16 00:00
PHST- 1998/12/16 00:00 [pubmed]
PHST- 1998/12/16 00:01 [medline]
PHST- 1998/12/16 00:00 [entrez]
PHST- 1999/01/01 00:00 [pmc-release]
AID - 1274 [pii]
AID - 10.1128/JVI.73.1.325-333.1999 [doi]
PST - ppublish
SO  - J Virol. 1999 Jan;73(1):325-33. doi: 10.1128/JVI.73.1.325-333.1999.