PMID- 9850993
OWN - NLM
STAT- MEDLINE
DCOM- 19990204
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 63
IP  - 2-4
DP  - 1998 Oct
TI  - Monoclonal antibodies against conformationally dependent epitopes on porcine 
      reproductive and respiratory syndrome virus.
PG  - 125-36
AB  - Monoclonal antibodies (MAbs) against porcine reproductive and respiratory 
      syndrome virus (PRRSV) were prepared and characterized. Four MAbs were developed 
      from the mice immunized with the recombinant GP4 protein expressed in insect 
      cells, and six MAbs were derived from the immunization with recombinant GP5 
      protein. All of the MAbs showed strong perinuclear fluorescence in PRRSV VR2385 
      infected cells by immunofluorescence staining. Among the MAbs to GP5 protein, one 
      showed strong reactivity in ELISA and recognized a 26 kDa band of PRRSV in a 
      western blot assay, while another showed neutralizing activity against the VR2385 
      isolate. Out of the four MAbs to GP4 protein, one showed mild reactivity in ELISA 
      with detergent extracted antigen, but had no reactivity in a western-blot assay. 
      The failure of MAb binding to detergent extracted antigen in ELISA or in 
      western-blot analysis indicated that the MAbs were against conformationally 
      dependent epitopes. Reactivity patterns of the MAbs with PRRSV field isolates 
      tested by fixed-cell ELISA showed that there are antigenic variations in PRRSV 
      GP4 and GP5 proteins. Development of these MAbs will benefit further studies on 
      PRRSV structural proteins as well as in understanding their roles in PRRSV 
      pathogenesis.
FAU - Zhang, Y
AU  - Zhang Y
AD  - Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State 
      University, Ames 50011, USA.
FAU - Sharma, R D
AU  - Sharma RD
FAU - Paul, P S
AU  - Paul PS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - *Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Antigens, Viral/*analysis/*immunology
MH  - Cell Line
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/*analysis/chemistry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Mice
MH  - Open Reading Frames
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/*isolation & purification
MH  - Recombinant Proteins/immunology
MH  - Sensitivity and Specificity
EDAT- 1998/12/16 00:00
MHDA- 1998/12/16 00:01
CRDT- 1998/12/16 00:00
PHST- 1998/12/16 00:00 [pubmed]
PHST- 1998/12/16 00:01 [medline]
PHST- 1998/12/16 00:00 [entrez]
AID - S0378-1135(98)00231-4 [pii]
AID - 10.1016/s0378-1135(98)00231-4 [doi]
PST - ppublish
SO  - Vet Microbiol. 1998 Oct;63(2-4):125-36. doi: 10.1016/s0378-1135(98)00231-4.