PMID- 9850997
OWN - NLM
STAT- MEDLINE
DCOM- 19990204
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 63
IP  - 2-4
DP  - 1998 Oct
TI  - Influence of pig age on virus titer and bactericidal activity of porcine 
      reproductive and respiratory syndrome virus (PRRSV)-infected pulmonary 
      intravascular macrophages (PIMs).
PG  - 177-87
AB  - Twelve pigs (six 4-week-old and six 4-month-old cross-bred, specific pathogen 
      free pigs) were used as donors for both pulmonary intravascular macrophages 
      (PIMs) and pulmonary alveolar macrophages (PAMs). The PIMs and PAMs were infected 
      in vitro with low (ISU-55) or high (VR-2385) virulence strains of PRRSV at 1 
      multiplicity of infection (m.o.i.) for comparisons of virus titers at 48 h post 
      infection (PI). PIMs were as permissive as PAMs to infection with both PRRSV 
      isolates yielding similar progeny titers (10(4.81) vs. 10(5.22) TCID50/ml, 
      respectively). Both ISU-55 and VR-2385 were able to infect PIMs and no 
      significant difference in virus replication as measured by virus titers between 
      isolates was found (10(5.33) vs. 10(4.69) TCID50/ml, respectively). PIMs from 
      4-weak-old pigs yielded a higher virus titer following PRRSV infection than PIMs 
      from 4-month-old pigs (10(5.43) vs. 10(4.59) TCID50/ml, respectively; p < 0.02). 
      VR-2385-infected PIMs had significantly decreased bactericidal (Staphylococcus 
      aureus) activity compared with uninfected PIMS at 48 h PI (p < 0.05). There was 
      no difference in bactericidal activity between ISU-55 (low virulence)-infected 
      PIMs and VR-2385 (high virulence)-infected PIMs. Both ISU-55 and VR-2385 
      infection significantly decreased the production of superoxide anion (SOA) at 24 
      and 48 h PI (p < 0.01). In conclusion, (1) PRRSV had a detrimental effect on 
      bactericidal activity and SOA production of PIMs, (2) PIMs from younger pigs were 
      more permissive to PRRSV infection, and (3) the selected PRRSV strains, which 
      differ in their abilities to induce pneumonia in vivo were not different when 
      tested in vitro by measuring virus titer and bactericidal functions.
FAU - Thanawongnuwech, R
AU  - Thanawongnuwech R
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames 50011, USA. roongroje.t@chula.ac.th
FAU - Thacker, E L
AU  - Thacker EL
FAU - Halbur, P G
AU  - Halbur PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Aging
MH  - Analysis of Variance
MH  - Animals
MH  - Blood Bactericidal Activity
MH  - Cells, Cultured
MH  - Immunity, Innate
MH  - Lung/virology
MH  - Macrophages/*physiology/ultrastructure/*virology
MH  - Macrophages, Alveolar/physiology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*physiopathology
MH  - Porcine respiratory and reproductive syndrome virus/pathogenicity/*physiology
MH  - Staphylococcus aureus
MH  - Swine
EDAT- 1998/12/16 00:00
MHDA- 1998/12/16 00:01
CRDT- 1998/12/16 00:00
PHST- 1998/12/16 00:00 [pubmed]
PHST- 1998/12/16 00:01 [medline]
PHST- 1998/12/16 00:00 [entrez]
AID - S0378-1135(98)00245-4 [pii]
AID - 10.1016/s0378-1135(98)00245-4 [doi]
PST - ppublish
SO  - Vet Microbiol. 1998 Oct;63(2-4):177-87. doi: 10.1016/s0378-1135(98)00245-4.