PMID- 9852618 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 0941-2905 (Print) IS - 0941-2905 (Linking) VI - 6 IP - 4 DP - 1998 Dec TI - Continuous and simultaneous cultivation of benthic food diatom Nitzschia sp. and abalone Haliotis sieboldii by using deep seawater. PG - 237-240 AB - By using low-temperature, clean, and nutrient-rich properties of deep seawater (DSW; seawater below the euphotic layer), a continuous and simultaneous cultivation system for a benthic food diatom, Nitzschia sp., and juvenile abalone was established. Cell suspension of Nitzschia sp. was added to a bioreactor made of acrylic pipe (7 cm diameter x 50 cm long) containing short vinyl tubes (2 cm diameter x 2 cm long) as substrata. DSW collected from 320 m depth at Muroto City, in Kochi Prefecture, Japan, was supplied to the reactor and incubated under natural light (ca. 6000 lux) with a continuous DSW flow rate of 40 turnovers per hour. After growing enough benthic diatoms in the reactor, juveniles of abalone, Haliotis sieboldii (shell length ca. 10-20 mm) were put into a reactor, and cultivated simultaneously with food diatoms in the continuous flow system. During the four-month incubation, 7-month-old abalone juveniles with a shell length of 12.4 (average) +/-0.2 (SD) mm were grown to 19.4 (+/-1.7) mm in the reactor. Daily growth rates of abalones were 50-110 microm/day. These results indicate that the continuous cultivation system with DSW supports the growth of juvenile abalone without any supply of seaweed until it grows to release size. FAU - Fukami, K AU - Fukami K AD - Laboratory of Aquatic Environmental Science (LAQUES), Kochi University, Nankoku-shi, Kochi 783-8502, Japan FAU - Kawai, A AU - Kawai A FAU - Asada, M AU - Asada M FAU - Okabe, M AU - Okabe M FAU - Hotta, T AU - Hotta T FAU - Moriyama, T AU - Moriyama T FAU - Doi, S AU - Doi S FAU - Nishijima, T AU - Nishijima T FAU - Yamaguchi, M AU - Yamaguchi M FAU - Taniguchi, M AU - Taniguchi M LA - eng PT - Journal Article PL - United States TA - J Mar Biotechnol JT - Journal of marine biotechnology JID - 9309765 EDAT- 1999/05/18 00:00 MHDA- 1999/05/18 00:01 CRDT- 1999/05/18 00:00 PHST- 1999/05/18 00:00 [pubmed] PHST- 1999/05/18 00:01 [medline] PHST- 1999/05/18 00:00 [entrez] AID - MB316 [pii] PST - ppublish SO - J Mar Biotechnol. 1998 Dec;6(4):237-240.