PMID- 9853116
OWN - NLM
STAT- MEDLINE
DCOM- 19990120
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 809
IP  - 2
DP  - 1998 Nov 2
TI  - Neuronal nicotinic acetylcholine receptors in rat trigeminal ganglia.
PG  - 238-45
AB  - The application of nicotine to the various epithelia served by the trigeminal 
      nerve produces irritation and/or pain by activating neuronal nicotinic 
      acetylcholine receptors (NnAChRS) in sensory neurons. In this study the NnAChRs 
      were identified in rat trigeminal ganglia (TG) using RT-PCR and 
      immunocytochemistry. With RT-PCR the subunits of NnAChRs in rat TG were 
      determined, and with immunocytochemistry the localization of three prominent 
      subunits (alpha 7, alpha 4 and beta 2) were localized in intact TG neurons. The 
      relative abundance of the alpha and beta subunits were: alpha 7 approximately 
      alpha 3 > alpha 6 > alpha 4 approximately alpha 5 > alpha 9 > or = alpha 2, and 
      beta 2 approximately beta 3 > beta 4. This is the first report of the alpha 9 
      subunit in TG. Immunohistochemical studies revealed that almost all TG neurons 
      contained alpha 7-LI and alpha 4-LI, and that 85% had beta 2-LI. For these three 
      subunits much of the label was internalized. Immunocytochemical studies using 
      antibodies raised against chick alpha 8 subunits did not specifically label rat 
      TG. These data reveal that rat TG neurons contain the entire spectrum of 
      mammalian NnAChR subunits.
FAU - Liu, L
AU  - Liu L
AD  - Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, 
      USA.
FAU - Chang, G Q
AU  - Chang GQ
FAU - Jiao, Y Q
AU  - Jiao YQ
FAU - Simon, S A
AU  - Simon SA
LA  - eng
GR  - DC 01065/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Chrna7 protein, rat)
RN  - 0 (DNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nicotinic)
RN  - 0 (alpha7 Nicotinic Acetylcholine Receptor)
SB  - IM
MH  - Animals
MH  - DNA Probes
MH  - Female
MH  - Gene Expression/physiology
MH  - Male
MH  - Neuritis/complications/*physiopathology
MH  - Neurons, Afferent/*chemistry
MH  - Pain/etiology/physiopathology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptors, Nicotinic/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trigeminal Nuclei/*chemistry/*cytology
MH  - alpha7 Nicotinic Acetylcholine Receptor
EDAT- 1998/12/16 00:00
MHDA- 1998/12/16 00:01
CRDT- 1998/12/16 00:00
PHST- 1998/12/16 00:00 [pubmed]
PHST- 1998/12/16 00:01 [medline]
PHST- 1998/12/16 00:00 [entrez]
AID - S0006-8993(98)00862-2 [pii]
AID - 10.1016/s0006-8993(98)00862-2 [doi]
PST - ppublish
SO  - Brain Res. 1998 Nov 2;809(2):238-45. doi: 10.1016/s0006-8993(98)00862-2.