PMID- 9855009
OWN - NLM
STAT- MEDLINE
DCOM- 19981223
LR  - 20190512
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 19
IP  - 11
DP  - 1998 Nov
TI  - Increased aneusomy and long arm deletion of chromosomes 5 and 7 in the 
      lymphocytes of Chinese workers exposed to benzene.
PG  - 1955-61
AB  - Two of the most common cytogenetic changes in therapy- and chemical-related 
      leukemia are the loss and long (q) arm deletion of chromosomes 5 and 7. The 
      detection of these aberrations in lymphocytes of individuals exposed to potential 
      leukemogens may serve as useful biomarkers of increased leukemia risk. We have 
      used a novel fluorescence in situ hybridization (FISH) procedure to determine if 
      specific aberrations in chromosomes 1, 5 and 7 occur at an elevated rate in the 
      blood cells of workers exposed to benzene. Forty-three healthy workers exposed to 
      a wide range of benzene concentrations (median 31 p.p.m., 8 h time-weighted 
      average) and 44 unexposed controls from Shanghai were studied. Whole blood was 
      cultured and metaphase spreads were harvested at 72 h. Benzene exposure was 
      associated with increases in the rates of monosomy 5 and 7 but not monosomy 1 (P 
      < 0.001, P < 0.0001 and P = 0.94, respectively) and with increases in trisomy and 
      tetrasomy frequencies of all three chromosomes. Long arm deletion of chromosomes 
      5 and 7 was increased in a dose-dependent fashion (P = 0.014 and P < 0.0001) up 
      to 3.5-fold in the exposed workers. These results demonstrate that 
      leukemia-specific changes in chromosomes 5 and 7 can be detected by FISH in the 
      peripheral blood of otherwise healthy benzene-exposed workers. We suggest that 
      aberrations in chromosomes 5 and 7 may be useful biomarkers of early biological 
      effect for benzene exposure.
FAU - Zhang, L
AU  - Zhang L
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California, Berkeley 94720-7360, USA.
FAU - Rothman, N
AU  - Rothman N
FAU - Wang, Y
AU  - Wang Y
FAU - Hayes, R B
AU  - Hayes RB
FAU - Li, G
AU  - Li G
FAU - Dosemeci, M
AU  - Dosemeci M
FAU - Yin, S
AU  - Yin S
FAU - Kolachana, P
AU  - Kolachana P
FAU - Titenko-Holland, N
AU  - Titenko-Holland N
FAU - Smith, M T
AU  - Smith MT
LA  - eng
GR  - P42 ES004705/ES/NIEHS NIH HHS/United States
GR  - P30 ES01896/ES/NIEHS NIH HHS/United States
GR  - P42 ES04705/ES/NIEHS NIH HHS/United States
GR  - R01 ES06721/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - J64922108F (Benzene)
SB  - IM
MH  - Adult
MH  - Benzene/*adverse effects
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 5
MH  - *Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - Leukemia, Myeloid, Acute/chemically induced/genetics
MH  - Lymphocytes/*ultrastructure
MH  - Male
MH  - Middle Aged
MH  - Monosomy
MH  - Occupational Exposure/*adverse effects
MH  - Trisomy
EDAT- 1998/12/17 00:00
MHDA- 1998/12/17 00:01
CRDT- 1998/12/17 00:00
PHST- 1998/12/17 00:00 [pubmed]
PHST- 1998/12/17 00:01 [medline]
PHST- 1998/12/17 00:00 [entrez]
AID - 10.1093/carcin/19.11.1955 [doi]
PST - ppublish
SO  - Carcinogenesis. 1998 Nov;19(11):1955-61. doi: 10.1093/carcin/19.11.1955.