PMID- 9855214
OWN - NLM
STAT- MEDLINE
DCOM- 19981231
LR  - 20190909
IS  - 0300-9742 (Print)
IS  - 0300-9742 (Linking)
VI  - 27
IP  - 6
DP  - 1998
TI  - Low copy numbers of human T-cell lymphotropic virus type I (HTLV-I) tax-like DNA 
      detected in the salivary gland of seronegative patients with Sjogren's syndrome 
      in an HTLV-I endemic area.
PG  - 435-40
AB  - To evaluate the hypothesis, proposed in previous reports from HTLV-I non-endemic 
      areas, that HTLV-I is involved in a significant proportion, about a quarter, of 
      Sjogren's syndrome patients who lack serum antibodies to the virus, we examined 
      for the presence or absence of HTLV-I in DNA samples isolated from salivary gland 
      tissues of 17 seronegative as well as 7 seropositive patients with Sjogren's 
      syndrome in Nagasaki, Japan, where the virus is highly endemic. The nested 
      two-step polymerase chain reaction (PCR), with a sensitivity capable of detecting 
      a single DNA molecule, failed to amplify the HTLV-I tax sequence from DNA of 14 
      of the 17 seronegative patients. The tax was only amplifiable from the tissue DNA 
      of the remaining three seronegative patients. The detection rate, 3/17 (18%), 
      was, unexpectedly, less than those previously reported from the HTLV-I 
      non-endemic areas. Moreover, in contrast to high viral loads (10(-1) to 10(-3) 
      per cell) in the salivary gland of the seropositive patients, a semiquantitative 
      PCR revealed that the copy number of the HTLV-I tax in the gland tissue of these 
      seronegative patients was very low, 10(-5) per cell. This level is unlikely to be 
      sufficient to promote an inflammatory reaction in the tissue. Our findings might 
      argue against the involvement of "prototype" HTLV-I in the pathogenesis of 
      Sjogren's syndrome in seronegative patients.
FAU - Mizokami, A
AU  - Mizokami A
AD  - Department of Bacteriology, Nagasaki University School of Medicine, Japan.
FAU - Eguchi, K
AU  - Eguchi K
FAU - Moriuchi, R
AU  - Moriuchi R
FAU - Futsuki, Y
AU  - Futsuki Y
FAU - Terada, K
AU  - Terada K
FAU - Nakamura, H
AU  - Nakamura H
FAU - Miyamoto, T
AU  - Miyamoto T
FAU - Katamine, S
AU  - Katamine S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Rheumatol
JT  - Scandinavian journal of rheumatology
JID - 0321213
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, tax)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - DNA Primers/chemistry
MH  - DNA, Viral/*analysis
MH  - Endemic Diseases
MH  - Female
MH  - Gene Dosage
MH  - Gene Products, tax/chemistry/*genetics
MH  - HTLV-I Infections/epidemiology
MH  - Human T-lymphotropic virus 1/*isolation & purification
MH  - Humans
MH  - Japan/epidemiology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Proviruses/chemistry
MH  - Salivary Glands/*virology
MH  - Sjogren's Syndrome/*virology
EDAT- 1998/12/17 00:00
MHDA- 1998/12/17 00:01
CRDT- 1998/12/17 00:00
PHST- 1998/12/17 00:00 [pubmed]
PHST- 1998/12/17 00:01 [medline]
PHST- 1998/12/17 00:00 [entrez]
AID - 10.1080/030097498442262 [doi]
PST - ppublish
SO  - Scand J Rheumatol. 1998;27(6):435-40. doi: 10.1080/030097498442262.