PMID- 9856081
OWN - NLM
STAT- MEDLINE
DCOM- 19990104
LR  - 20200324
IS  - 0304-8608 (Print)
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 143
IP  - 10
DP  - 1998
TI  - A nonstructural and antigenic glycoprotein is encoded by ORF3 of the IAF-Klop 
      strain of porcine reproductive and respiratory syndrome virus.
PG  - 1927-40
AB  - Open reading frame 3 (ORF3) of the genome of porcine reproductive and respiratory 
      syndrome virus (PRRSV), Quebec strain IAF-Klop, was reverse-transcribed and 
      cloned into the procaryotic expression vector pGEX-4T-1, then subcloned into the 
      eucaryotic expression vector pAdCMV5 which was used as a shuttle vector to 
      generate a replication-defective recombinant adenovirus. The procaryotic GST-ORF3 
      recombinant fusion protein was used to raise a monospecific antiserum in rabbits. 
      By Western-immunoblotting with PRRSV-infected cell extracts, the ORF3 encoded 
      protein had an estimated molecular mass (M(r)) of 42 kDa, similar to that of the 
      protein expressed by the adenovirus vector. Endoglycosidase F digestion showed 
      that the ORF3 encoded protein occurs in an highly glycosylated form (GP3) in the 
      infected MARC-145 cells. Pulse-chase and radioimmunoprecipitation experiments 
      revealed that the GP3 protein was present in amounts equivalent to those of the 
      N, M, and GP5 proteins in the infected cells, whereas no GP3 could be detected in 
      purified virions. During the first 30 min of chase, the GP3 undergoes a gradual 
      downward shift of its apparent M(r), thought to result from trimming of the 
      mannose-rich glycan structures. Tested convalescent pig sera that were found to 
      be seropositive to PRRSV by indirect immunofluorescence reacted positively with 
      the recombinant GST-ORF3 fusion protein by immunoblotting. Data indicated that 
      the ORF3 protein of the Quebec reference strain of PRRSV is a highly glycosylated 
      and antigenic protein, which is nonstructural.
FAU - Gonin, P
AU  - Gonin P
AD  - Center for Research in Virology, Armand-Frappier Institute, University of Quebec, 
      Laval, Canada.
FAU - Mardassi, H
AU  - Mardassi H
FAU - Gagnon, C A
AU  - Gagnon CA
FAU - Massie, B
AU  - Massie B
FAU - Dea, S
AU  - Dea S
LA  - eng
SI  - GENBANK/AF003344
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Antigens, Viral/*analysis/genetics/immunology
MH  - Glycosylation
MH  - Molecular Weight
MH  - *Open Reading Frames
MH  - Porcine respiratory and reproductive syndrome 
      virus/*chemistry/genetics/physiology
MH  - Rabbits
MH  - Swine
MH  - Viral Nonstructural Proteins/*analysis/genetics/immunology
MH  - Virus Assembly
PMC - PMC7086821
EDAT- 1998/12/18 00:00
MHDA- 1998/12/18 00:01
PMCR- 2020/03/23
CRDT- 1998/12/18 00:00
PHST- 1998/12/18 00:00 [pubmed]
PHST- 1998/12/18 00:01 [medline]
PHST- 1998/12/18 00:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 81431927 [pii]
AID - 10.1007/s007050050430 [doi]
PST - ppublish
SO  - Arch Virol. 1998;143(10):1927-40. doi: 10.1007/s007050050430.