PMID- 9856348
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20240315
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 8
IP  - 5
DP  - 1998 Oct 31
TI  - No effect of expression of neuron-restrictive silencer factor (NRSF) protein on 
      N-type Ca2+ channel alpha 1B gene promoter activity in NS20Y cells.
PG  - 600-5
AB  - The expression of the N-type voltage-sensitive calcium channel alpha1B gene is 
      restricted to neurons by an 5'-upstream region (-3992 to -1788) containing 
      negative regulation element(s) active in non-neuronal cells (Kim et al., 1997). 
      The neuron-restrictive silencer factor (NRSF) represses the transcription of 
      several neuronal genes in non-neuronal cells by binding to a 21 bp DNA element, 
      termed the neuron-restrictive silencer element (NRSE). To analyze the involvement 
      of NRSF in the neuron-specific expression of the alpha1B gene, the coding region 
      of NRSF cDNA was cloned by PCR and the exogenous NRSF cDNA was transiently 
      cotransfected with tester plasmids into NS20Y neuronal cells which do not contain 
      the endogenous NRSF protein. The luciferase activity of a positive control 
      plasmid NRSEL containing a single copy of the NRSE sequence of the SCG10 gene was 
      repressed 5 fold in HeLa cells containing the endogenous NRSF protein, and its 
      activity was repressed to 44-27% of the control with an increasing amount of 
      exogenous DNA in NS20Y cells. Unlike NRSEL, the promoter activity of the alpha1B 
      subunit-luciferase fusion construct (-3992L) was about 15 fold repressed in HeLa 
      cells compared to NS20Y cells, while any remarkable changes was undetectable in 
      the NRSF expressed NS20Y. These results suggest that the repression of the 
      alpha1B gene in non-neuronal cells may not be mediated by the NRSF function.
FAU - Kim, D S
AU  - Kim DS
AD  - Department of Anatomy, School of Medicine, Kyungpook National University, Taegu, 
      Korea. doskim@kyungpook.ac.kr
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (Calcium Channels)
RN  - 0 (DNA, Complementary)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Calcium Channels/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - HeLa Cells
MH  - Humans
MH  - Neuroblastoma/genetics/pathology
MH  - *Promoter Regions, Genetic
MH  - Repressor Proteins/*genetics
MH  - *Transcription Factors
MH  - Tumor Cells, Cultured
EDAT- 1998/12/18 00:00
MHDA- 1998/12/18 00:01
CRDT- 1998/12/18 00:00
PHST- 1998/12/18 00:00 [pubmed]
PHST- 1998/12/18 00:01 [medline]
PHST- 1998/12/18 00:00 [entrez]
AID - S1016-8478(23)13471-6 [pii]
PST - ppublish
SO  - Mol Cells. 1998 Oct 31;8(5):600-5.