PMID- 9856820
OWN - NLM
STAT- MEDLINE
DCOM- 19990104
LR  - 20181201
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 111
IP  - 6
DP  - 1998 Dec
TI  - Increased type 2 cytokine expression by both CD4+ CD45RO+ T cells and CD8+ 
      CD45RO+ T cells in blood circulation is associated with high serum IgE but not 
      with atopic dermatitis.
PG  - 1079-84
AB  - Type 2 cytokines, such as interleukin-4 (IL-4) and IL-13, are associated with 
      immunoglobulin E (IgE) production. This association has also been observed in 
      CD8+ T cells from patients infected with leprosy and human immunodeficiency virus 
      (HIV). Using intracellular cytokine staining and flow cytometry, the cytokine 
      profile [IL-2, IL-4, IL-10, IL-13, and interferon (IFN)-gamma] of both CD4+ and 
      CD8+ memory/effector T cells circulating in atopic dermatitis (AD) patients was 
      investigated at the single cell level. The levels of type 2 cytokines in CD4+ T 
      cells or CD8+ T cells in AD patients with high levels of serum IgE (AD-H), low 
      levels of serum IgE (AD-L), and healthy controls were compared. Increased 
      production of IL-4 and IL-13 in both CD4+ CD45RO+ T cells and CD8+ CD45RO+ T 
      cells after 4 h in vitro stimulation with phorbol 12-myristate 13-acetate and 
      ionomycin, was more prominent in AD-H patients than in AD-L patients or healthy 
      controls, whereas IFN-gamma-producing CD4+ CD45RO+ T cells and CD8+ CD45RO+ T 
      cells were relatively diminished in AD-H patients. CD4+ T cells and CD8 + T cells 
      from AD-H patients, cultured for 48 h with phorbol 12-myristate 13-acetate and 
      ionomycin, released larger amounts of IL-4 and IL-13 but smaller amounts of 
      IFN-gamma than both types of cells from AD-L patients or healthy controls. In 
      addition, when stimulated with immobilized anti-CD3 monoclonal antibody (MoAb) 
      and anti-CD28 MoAb, CD4+ CD45RO+ T cells and CD8+ CD45RO+ T cells from AD-H 
      patients contained more IL-4-producing cells but fewer IFN-gamma-producing cells 
      compared with healthy controls. Finally, spontaneous mRNA expression of IL-4 in 
      blood CD8+ CD45RO+ T cells isolated from AD-H patients was increased, as 
      determined by reverse transcriptase-polymerase chain reaction. Therefore, in AD 
      patients with high IgE levels, type 2 cytokine (IL-4 and IL-13) expression is 
      associated with IgE production, in both CD4+ CD45RO+ T cell and CD8+ CD45RO+ T 
      cell subsets.
FAU - Sato, A
AU  - Sato A
AD  - Department of Dermatology, Okayama University Medical School, Japan.
FAU - Tsuji, K
AU  - Tsuji K
FAU - Yamamura, M
AU  - Yamamura M
FAU - Morita, Y
AU  - Morita Y
FAU - Kanzaki, H
AU  - Kanzaki H
FAU - Tada, J
AU  - Tada J
FAU - Makino, H
AU  - Makino H
FAU - Arata, J
AU  - Arata J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Interleukin-13)
RN  - 0 (RNA, Messenger)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Blood Circulation/immunology
MH  - CD4-Positive T-Lymphocytes/*metabolism
MH  - CD8-Positive T-Lymphocytes/*metabolism
MH  - Cells, Cultured
MH  - Child
MH  - Dermatitis, Atopic/*blood
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Interferon-gamma/blood/metabolism
MH  - Interleukin-13/*biosynthesis/blood/metabolism
MH  - Interleukin-4/*biosynthesis/genetics/metabolism
MH  - Male
MH  - RNA, Messenger/metabolism
EDAT- 1998/12/18 00:00
MHDA- 1998/12/18 00:01
CRDT- 1998/12/18 00:00
PHST- 1998/12/18 00:00 [pubmed]
PHST- 1998/12/18 00:01 [medline]
PHST- 1998/12/18 00:00 [entrez]
AID - S0022-202X(15)40321-5 [pii]
AID - 10.1046/j.1523-1747.1998.00454.x [doi]
PST - ppublish
SO  - J Invest Dermatol. 1998 Dec;111(6):1079-84. doi: 
      10.1046/j.1523-1747.1998.00454.x.