PMID- 9858220
OWN - NLM
STAT- MEDLINE
DCOM- 19990127
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 103
IP  - 3
DP  - 1998 Dec
TI  - Chromosomal abnormalities in systemic amyloidosis.
PG  - 704-10
AB  - Primary systemic amyloidosis (AL) is a plasma cell disorder characterized by 
      deposition of monoclonal light chains in different organ systems. Although 
      multiple and complex numerical chromosomal abnormalities have been described in 
      patients with multiple myeloma, it is currently unknown whether such changes 
      occur in systemic amyloidosis. Bone marrow samples from 21 patients with AL were 
      studied by standard cytogenetics and interphase fluorescence in situ 
      hybridization (FISH) for the presence of numerical chromosomal abnormalities. We 
      tested for six chromosomes (7, 11, 9, 15, 18 and X) using centromere-specific 
      probes. The monoclonal plasma cells were identified by simultaneous fluorescent 
      staining of the monotypic cytoplasmic immunoglobulin. We compared these results 
      with those obtained from 19 patients with monoclonal gammopathy of undetermined 
      significance (MGUS) and normal controls. Multiple numerical chromosomal 
      abnormalities were detected in AL by interphase FISH, including trisomy of 
      chromosomes 7 (42%), 9 (52%), 11 (47%), 15 (39%), 18 (33%) and X (13% in women 
      and 54% in men). Monosomy of chromosome 18 was seen in 72% of cases. Previous 
      exposure to alkylator therapy did not appear to correlate with these 
      abnormalities. No significant difference was observed in the prevalence of these 
      abnormalities between AL and MGUS. Multiple chromosomal numerical abnormalities 
      were detected by interphase FISH analysis in patients with AL, especially 
      monosomy of chromosome 18. Aneuploidy in the monotypic plasma supports a 
      neoplastic nature for the disorder.
FAU - Fonseca, R
AU  - Fonseca R
AD  - Division of Hematology and Internal Medicine, Mayo Clinic and Mayo Foundation, 
      Rochester, Minnesota 55905, USA.
FAU - Ahmann, G J
AU  - Ahmann GJ
FAU - Jalal, S M
AU  - Jalal SM
FAU - Dewald, G W
AU  - Dewald GW
FAU - Larson, D R
AU  - Larson DR
FAU - Therneau, T M
AU  - Therneau TM
FAU - Gertz, M A
AU  - Gertz MA
FAU - Kyle, R A
AU  - Kyle RA
FAU - Greipp, P R
AU  - Greipp PR
LA  - eng
GR  - CA62242/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Amyloidosis/*genetics
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 15/genetics
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Chromosomes, Human, Pair 7/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Male
MH  - Middle Aged
MH  - Paraproteinemias/genetics
MH  - X Chromosome/genetics
EDAT- 1998/12/19 00:00
MHDA- 1998/12/19 00:01
CRDT- 1998/12/19 00:00
PHST- 1998/12/19 00:00 [pubmed]
PHST- 1998/12/19 00:01 [medline]
PHST- 1998/12/19 00:00 [entrez]
AID - 10.1046/j.1365-2141.1998.01034.x [doi]
PST - ppublish
SO  - Br J Haematol. 1998 Dec;103(3):704-10. doi: 10.1046/j.1365-2141.1998.01034.x.