PMID- 9858479
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20190512
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 60
IP  - 1
DP  - 1999 Jan
TI  - Production of gelatinases and tissue inhibitors of matrix metalloproteinases by 
      equine ovarian stromal cells In vitro.
PG  - 1-7
AB  - Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) play very 
      important roles in extracellular matrix (ECM) remodeling in ovarian follicle 
      growth and ovulation. Equine follicles are embedded in cortex that is at the 
      center of the ovary, and they must expand/emigrate to the fossa, the only site in 
      the ovary for ovulation. Therefore, equine ovarian stromal cells (EOSC) are 
      probably involved in ECM remodeling during follicle growth. This study examined 
      whether cultured EOSC synthesize gelatinases and TIMPs, molecules essential for 
      ECM remodeling in other systems. Results showed that cultured EOSC (passage 3-8) 
      had a fibroblast-like morphology and were positive for alpha-smooth muscle actin 
      and type I procollagen by immunostaining. Gelatinase A (MMP-2), gelatinase B 
      (MMP-9), TIMP-1, and TIMP-2 were present in EOSC-conditioned medium, and TIMP-3 
      in ECM of EOSC. Transforming growth factor beta significantly stimulated the 
      activity of gelatinases A and B and TIMP-1 in conditioned medium from EOSC (p < 
      0.05). Phorbol 12-myristate 13-acetate also significantly stimulated the activity 
      of gelatinases A and B and TIMP-1 in conditioned medium and of TIMP-3 in ECM (p < 
      0.05). Our results suggest that EOSC produce important components of the ECM 
      remodeling machinery and, therefore, may play a role in the ECM remodeling during 
      follicle growth in this species.
FAU - Song, L
AU  - Song L
AD  - a Department of Biomedical Sciences, Ontario Veterinary College, University of 
      Guelph, Guelph, Ontario, Canada N1G 2W1.
FAU - Porter, D G
AU  - Porter DG
FAU - Coomber, B L
AU  - Coomber BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (Transforming Growth Factor beta)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Collagenases/biosynthesis
MH  - Culture Media, Conditioned
MH  - Dose-Response Relationship, Drug
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Gelatinases/*biosynthesis
MH  - Horses/*metabolism
MH  - Matrix Metalloproteinase 2
MH  - Matrix Metalloproteinase 9
MH  - Metalloendopeptidases/biosynthesis
MH  - Ovary/*metabolism
MH  - Stromal Cells/*metabolism
MH  - Tetradecanoylphorbol Acetate/administration & dosage/pharmacology
MH  - Tissue Inhibitor of Metalloproteinase-1/biosynthesis
MH  - Tissue Inhibitor of Metalloproteinase-2/biosynthesis
MH  - Tissue Inhibitor of Metalloproteinases/*biosynthesis
MH  - Transforming Growth Factor beta/administration & dosage/pharmacology
EDAT- 1998/12/22 00:00
MHDA- 1998/12/22 00:01
CRDT- 1998/12/22 00:00
PHST- 1998/12/22 00:00 [pubmed]
PHST- 1998/12/22 00:01 [medline]
PHST- 1998/12/22 00:00 [entrez]
AID - 10.1095/biolreprod60.1.1 [doi]
PST - ppublish
SO  - Biol Reprod. 1999 Jan;60(1):1-7. doi: 10.1095/biolreprod60.1.1.