PMID- 9858829
OWN - NLM
STAT- MEDLINE
DCOM- 19990201
LR  - 20180213
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 82
IP  - 3-4
DP  - 1998
TI  - Application of FISH for in situ detection and quantification of DNA breakage.
PG  - 251-6
AB  - We describe a simple procedure that allows the use of fluorescence in situ 
      hybridization (FISH) for in situ detection of DNA strand breaks in single cells 
      (DBD-FISH: DNA Breakage Detection-FISH). After trapping within an agarose 
      microgel, cells are incubated in an unwinding alkaline solution, deproteinized 
      and dehydrated. Areas of single-stranded DNA are generated by the alkaline 
      solution in proportion to the degree of DNA strand breakage. These then act as 
      targets for FISH of whole genomic or region-specific probes (telomeric, human 
      chromosome 8 painting, human alphoid DXZ1 locus, and human c-erbB-2 cosmid 
      probes). Measurement of the amount and surface of FISH signals provides 
      information on the breakage level in probed areas, permitting the assessment of 
      possible intragenomic differences in sensitivity as well as intercellular 
      heterogeneity in DNA damage induction or repair.
FAU - Fernandez, J L
AU  - Fernandez JL
AD  - Laboratorio de Genetica Molecular y Radiobiologia, Centro Oncologico de Galicia, 
      La Coruna (Spain). genetica<gcog@jet.es>
FAU - Goyanes, V J
AU  - Goyanes VJ
FAU - Ramiro-Diaz, J
AU  - Ramiro-Diaz J
FAU - Gosalvez, J
AU  - Gosalvez J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cell Nucleus/genetics/radiation effects
MH  - *Chromosomes, Human, Pair 8
MH  - Cricetinae
MH  - *DNA Damage
MH  - DNA Mutational Analysis/methods
MH  - Dose-Response Relationship, Radiation
MH  - Female
MH  - Genome, Human
MH  - Humans
MH  - Image Processing, Computer-Assisted/methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukocytes/radiation effects
EDAT- 1998/12/22 03:02
MHDA- 2000/08/16 11:00
CRDT- 1998/12/22 03:02
PHST- 1998/12/22 03:02 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1998/12/22 03:02 [entrez]
AID - ccg82251 [pii]
AID - 10.1159/000015112 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1998;82(3-4):251-6. doi: 10.1159/000015112.