PMID- 9860180
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20190921
IS  - 0920-5063 (Print)
IS  - 0920-5063 (Linking)
VI  - 9
IP  - 11
DP  - 1998
TI  - Retinal pigment epithelium cell culture on thin biodegradable 
      poly(DL-lactic-co-glycolic acid) films.
PG  - 1187-205
AB  - Thin films of 50:50 and 75:25 poly(DL-lactic-co-glycolic acid) (PLGA) were 
      manufactured with a controlled thickness of less than 10 microm. The effect of 
      PLGA copolymer ratio on in vitro cell attachment, proliferation, morphology, and 
      tight junction formation was evaluated using a human D407 retinal pigment 
      epithelium (RPE) cell line. Almost complete cell attachment was achieved on both 
      PLGA films after 8 h of cell seeding, which was comparable to that on tissue 
      culture polystyrene (TCPS) controls. The initial cell seeding density affected 
      attachment, and the optimal value for 50:50 PLGA was 25000 cells cm(-2). After 7 
      days of in vitro culture, cell density on 50:50 and 75:25 PLGA films increased 45 
      and 40 folds, respectively, and a 34-fold increase was observed on TCPS. The RPE 
      cells cultured on PLGA films at confluence had a characteristic cobblestone 
      morphology. Confluent RPE cells also developed normal tight junctions in vitro 
      which were concentrated mainly at the apical surfaces of cell-cell junctions. 
      These results demonstrated that thin biodegradable PLGA films can provide 
      suitable substrates for human RPE cell culture, and may serve as temporary 
      carriers for subretinal implantation of organized sheets of RPE.
FAU - Lu, L
AU  - Lu L
AD  - Department of Chemical Engineering and Institute of Biosciences and 
      Bioengineering, Rice University, Houston, TX 77251-1892, USA.
FAU - Garcia, C A
AU  - Garcia CA
FAU - Mikos, A G
AU  - Mikos AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biomater Sci Polym Ed
JT  - Journal of biomaterials science. Polymer edition
JID - 9007393
RN  - 0 (Biocompatible Materials)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Polymers)
RN  - 0 (TJP1 protein, human)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - *Biocompatible Materials
MH  - Biodegradation, Environmental
MH  - Cell Adhesion
MH  - Cell Culture Techniques/*instrumentation
MH  - Cell Division
MH  - Cells, Cultured
MH  - Humans
MH  - Intercellular Junctions
MH  - *Lactic Acid
MH  - Membrane Proteins/analysis/metabolism
MH  - Phosphoproteins/analysis/metabolism
MH  - Pigment Epithelium of Eye/*cytology
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers
MH  - Zonula Occludens-1 Protein
OTO - NASA
OT  - Non-programmatic
EDAT- 1998/12/22 00:00
MHDA- 1998/12/22 00:01
CRDT- 1998/12/22 00:00
PHST- 1998/12/22 00:00 [pubmed]
PHST- 1998/12/22 00:01 [medline]
PHST- 1998/12/22 00:00 [entrez]
AID - 10.1163/156856298x00721 [doi]
PST - ppublish
SO  - J Biomater Sci Polym Ed. 1998;9(11):1187-205. doi: 10.1163/156856298x00721.