PMID- 9861038
OWN - NLM
STAT- MEDLINE
DCOM- 19990128
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 95
IP  - 26
DP  - 1998 Dec 22
TI  - Modulation of N-methyl-D-aspartate receptor function by glycine transport.
PG  - 15730-4
AB  - The recent discovery of glycine transporters in both the central nervous system 
      and the periphery suggests that glycine transport may be critical to 
      N-methyl-D-aspartate receptor (NMDAR) function by controlling glycine 
      concentration at the NMDAR modulatory glycine site. Data obtained from whole-cell 
      patch-clamp recordings of hippocampal pyramidal neurons, in vitro, demonstrated 
      that exogenous glycine and glycine transporter type 1 (GLYT1) antagonist 
      selectively enhanced the amplitude of the NMDA component of a glutamatergic 
      excitatory postsynaptic current. The effect was blocked by 
      2-amino-5-phosphonovaleric acid and 7-chloro-kynurenic acid but not by 
      strychnine. Thus, the glycine-binding site was not saturated under the control 
      conditions. Furthermore, GLYT1 antagonist enhanced NMDAR function during 
      perfusion with medium containing 10 microM glycine, a concentration similar to 
      that in the cerebrospinal fluid in vivo, thereby supporting the hypothesis that 
      the GLYT1 maintains subsaturating concentration of glycine at synaptically 
      activated NMDAR. The enhancement of NMDAR function by specific GLYT1 antagonism 
      may be a feasible target for therapeutic agents directed toward diseases related 
      to hypofunction of NMDAR.
FAU - Bergeron, R
AU  - Bergeron R
AD  - Laboratory of Neuroscience, Department of Psychiatry, Harvard Medical School, 115 
      Mill Street, Belmont, MA 02178-9106, USA.
FAU - Meyer, T M
AU  - Meyer TM
FAU - Coyle, J T
AU  - Coyle JT
FAU - Greene, R W
AU  - Greene RW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Amino Acid Transport Systems, Neutral)
RN  - 0 (Carrier Proteins)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Glycine Plasma Membrane Transport Proteins)
RN  - 0 (Quinoxalines)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Slc6a9 protein, rat)
RN  - 62T278S1MX (FG 9041)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - TE7660XO1C (Glycine)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate/pharmacology
MH  - *Amino Acid Transport Systems, Neutral
MH  - Animals
MH  - Bicuculline/pharmacology
MH  - Carrier Proteins/antagonists & inhibitors/*physiology
MH  - Electric Stimulation
MH  - Excitatory Amino Acid Antagonists/*pharmacology
MH  - *Excitatory Postsynaptic Potentials/drug effects
MH  - Glycine/*physiology
MH  - Glycine Plasma Membrane Transport Proteins
MH  - Hippocampus/*physiology
MH  - In Vitro Techniques
MH  - Patch-Clamp Techniques
MH  - Pyramidal Cells/*physiology
MH  - Quinoxalines/pharmacology
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/*physiology
PMC - PMC28112
EDAT- 1998/12/23 00:00
MHDA- 1998/12/23 00:01
PMCR- 1999/06/22
CRDT- 1998/12/23 00:00
PHST- 1998/12/23 00:00 [pubmed]
PHST- 1998/12/23 00:01 [medline]
PHST- 1998/12/23 00:00 [entrez]
PHST- 1999/06/22 00:00 [pmc-release]
AID - 4050 [pii]
AID - 10.1073/pnas.95.26.15730 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15730-4. doi: 
      10.1073/pnas.95.26.15730.