PMID- 9862465
OWN - NLM
STAT- MEDLINE
DCOM- 19990114
LR  - 20190725
IS  - 0026-8925 (Print)
IS  - 0026-8925 (Linking)
VI  - 260
IP  - 2-3
DP  - 1998 Nov
TI  - The sugarless mutation affects the expression of the white eye color gene in 
      Drosophila melanogaster.
PG  - 131-43
AB  - Investigation of a modifier locus displaying a darker eye phenotype in 
      white-apricot flies led to the isolation of the gene encoding UDP-glucose 
      dehydrogenase (UDPGDH). The P-element insertion l(3)05007 occurs upstream of the 
      transcription start site of the sugarless (sgl) gene and greatly reduces its 
      transcription at various developmental stages. A single abundant sgl transcript 
      shows a ubiquitous distribution and encodes a 53-kDa protein which is 64% 
      identical in sequence to bovine UDP-glucose dehydrogenase. Overexpression of sgl 
      in E. coli resulted in synthesis of a protein with high levels of UDPGDH 
      activity. Expression of three genes that participate in pigment deposition, 
      white, scarlet and brown, was significantly affected in populations segregating 
      for sgl, suggesting that it is the decrease in UDPGDH level that produces the 
      modifying effect observed. In addition, genetic effects on white-apricot were 
      observed in sgl-wingless and sgl-hedgehog double mutants. Recent data have 
      indicated an effect of UDPGDH on cell surface glycosaminoglycans (GAGs) which 
      modulate the activity of growth factors, and in particular wingless signaling. 
      Our results suggest that the levels of GAGs are rate limiting for cell-cell 
      signaling pathways which mediate changes in gene expression.
FAU - Benevolenskaya, E V
AU  - Benevolenskaya EV
AD  - Division of Biological Sciences, University of Missouri-Columbia 65211, USA.
FAU - Frolov, M V
AU  - Frolov MV
FAU - Birchler, J A
AU  - Birchler JA
LA  - eng
SI  - GENBANK/AF001310
SI  - GENBANK/AF001311
SI  - GENBANK/AF001312
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Mol Gen Genet
JT  - Molecular & general genetics : MGG
JID - 0125036
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (bw protein, Drosophila)
RN  - 0 (st protein, Drosophila)
RN  - 0 (w protein, Drosophila)
RN  - EC 1.1.1.22 (Uridine Diphosphate Glucose Dehydrogenase)
SB  - IM
MH  - ATP-Binding Cassette Transporters/genetics/metabolism
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cattle
MH  - Cloning, Molecular
MH  - DNA Transposable Elements
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/*genetics/growth & development
MH  - Escherichia coli/genetics
MH  - Eye Proteins/*genetics/metabolism
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Suppressor
MH  - Insect Proteins/*genetics/metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Uridine Diphosphate Glucose Dehydrogenase/*genetics/metabolism
EDAT- 1998/12/23 00:00
MHDA- 1998/12/23 00:01
CRDT- 1998/12/23 00:00
PHST- 1998/12/23 00:00 [pubmed]
PHST- 1998/12/23 00:01 [medline]
PHST- 1998/12/23 00:00 [entrez]
AID - 10.1007/s004380050879 [doi]
PST - ppublish
SO  - Mol Gen Genet. 1998 Nov;260(2-3):131-43. doi: 10.1007/s004380050879.