PMID- 9862675
OWN - NLM
STAT- MEDLINE
DCOM- 19990122
LR  - 20211025
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 161
IP  - 12
DP  - 1998 Dec 15
TI  - Ox-40 ligand: a potent costimulatory molecule for sustaining primary CD4 T cell 
      responses.
PG  - 6510-7
AB  - Ox-40 and Ox-40 ligand (Ox-40L) are thought to be involved in T cell-APC 
      interactions. However, their exact role in T cell responses is undefined. Using 
      fibroblast transfectants expressing Ox-40L and/or B7-1, and CD4 cells from TCR 
      transgenic mice, we investigated the effect of Ox-40 signaling on primary 
      responses to the Ag pigeon cytochrome c. Ox-40 expression on naive CD4 cells 
      peaked 2 to 3 days after activation, and was lost by 4 to 5 days. APCs with 
      Ox-40L promoted partial activation of naive T cells with some IL-2 secretion, but 
      were unable to enhance proliferation, unlike those with B7-1. APCs coexpressing 
      Ox-40L with B7-1 induced large quantities of IL-2 and promoted proliferative 
      responses that persisted for several days. Effector cells taken 5 days after 
      naive T cell activation reexpressed Ox-40 within 4 h and responded strongly to 
      APCs expressing Ox-40L, whereas B7-1 had little effect. Synergy was also seen 
      between Ox-40L and B7-1, with primarily IL-2 being elevated, although IL-4 and 
      IL-5 were also up-regulated. The most striking action was on effector T cell 
      proliferation, which continued at high levels for up to 4 days, with little 
      proliferation evident at this time in the absence of Ox-40 signals. These data 
      suggest that Ox-40/Ox-40L interactions act after initial activation events to 
      prolong clonal expansion and enhance effector cytokine secretion, and may be 
      involved in promoting long-lived primary CD4 responses.
FAU - Gramaglia, I
AU  - Gramaglia I
AD  - Division of Immunochemistry, La Jolla Institute for Allergy and Immunology, San 
      Diego, CA 92121, USA.
FAU - Weinberg, A D
AU  - Weinberg AD
FAU - Lemon, M
AU  - Lemon M
FAU - Croft, M
AU  - Croft M
LA  - eng
GR  - AI36259/AI/NIAID NIH HHS/United States
GR  - NS35421/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (B7-1 Antigen)
RN  - 0 (CD28 Antigens)
RN  - 0 (Cytochrome c Group)
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukin-5)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (OX40 Ligand)
RN  - 0 (Peptide Fragments)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Receptors, OX40)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tnfrsf4 protein, mouse)
RN  - 0 (Tnfsf4 protein, mouse)
RN  - 0 (Tumor Necrosis Factor Receptor Superfamily, Member 7)
RN  - 0 (Tumor Necrosis Factors)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/*immunology
MH  - B7-1 Antigen/genetics/immunology
MH  - CD28 Antigens/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cells, Cultured
MH  - Columbidae
MH  - Cytochrome c Group/immunology
MH  - Fibroblasts
MH  - Interleukin-2/metabolism
MH  - Interleukin-4/metabolism
MH  - Interleukin-5/metabolism
MH  - Lymphocyte Activation/*physiology
MH  - *Membrane Glycoproteins
MH  - Mice
MH  - Mice, Transgenic
MH  - OX40 Ligand
MH  - Peptide Fragments/immunology
MH  - Receptors, Antigen, T-Cell/genetics
MH  - Receptors, OX40
MH  - Receptors, Tumor Necrosis Factor/genetics/*immunology
MH  - Recombinant Fusion Proteins/immunology
MH  - Transfection
MH  - Tumor Necrosis Factor Receptor Superfamily, Member 7/*immunology
MH  - Tumor Necrosis Factors
EDAT- 1998/12/23 00:00
MHDA- 1998/12/23 00:01
CRDT- 1998/12/23 00:00
PHST- 1998/12/23 00:00 [pubmed]
PHST- 1998/12/23 00:01 [medline]
PHST- 1998/12/23 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1998 Dec 15;161(12):6510-7.