PMID- 9862717
OWN - NLM
STAT- MEDLINE
DCOM- 19990122
LR  - 20201219
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 161
IP  - 12
DP  - 1998 Dec 15
TI  - Lymphotoxin alpha3 induces chemokines and adhesion molecules: insight into the 
      role of LT alpha in inflammation and lymphoid organ development.
PG  - 6853-60
AB  - Lymphotoxin (LT) plays an important role in inflammation and lymphoid organ 
      development, though the mechanisms by which it promotes these processes are 
      poorly understood. Toward this end, the biologic activities of a recently 
      generated recombinant murine (m) LT alpha preparation were evaluated. This 
      cytokine preparation was effective at inducing cytotoxicity of WEHI target cells 
      with 50% maximal killing observed with 1.2 ng/ml. mLT alpha also induced the 
      expression of inflammatory mediators in the murine endothelial cell line bEnd.3. 
      rmLT alpha induced expression of the adhesion molecules VCAM, ICAM, E-selectin, 
      and the mucosal addressin cellular adhesion molecule, MAdCAM-1. When mLT alpha, 
      human (h) LT alpha, and mTNF-alpha were compared, mLT alpha was the most potent 
      inducer of MAdCAM-1. None of these cytokines induced the peripheral node 
      addressin, PNAd. mLT alpha also induced expression of the chemokines RANTES, 
      IFN-inducible protein 10 (IP-10), and monocyte chemotactic protein 1 (MCP-1). 
      mRNA levels peaked 4 h following treatment with mLT alpha and declined through 
      the 24-h treatment period. LT alpha also induced chemokine protein within 8 h of 
      treatment, which increased through the 24-h treatment period. These data 
      demonstrate that the proinflammatory effects of LT alpha3 may be mediated in part 
      through the induction of adhesion molecule and chemokine expression. Further, LT 
      alpha3 may promote development of lymphoid tissue through induction of chemokines 
      and the mucosal addressin MAdCAM-1. These data confirm previous observations in 
      transgenic and knockout mice that LT alpha3 in the absence of LT beta carries out 
      unique biologic activities.
FAU - Cuff, C A
AU  - Cuff CA
AD  - Department of Epidemiology and Public Health, Yale University School of Medicine, 
      New Haven, CT 06520, USA.
FAU - Schwartz, J
AU  - Schwartz J
FAU - Bergman, C M
AU  - Bergman CM
FAU - Russell, K S
AU  - Russell KS
FAU - Bender, J R
AU  - Bender JR
FAU - Ruddle, N H
AU  - Ruddle NH
LA  - eng
GR  - 5 T32 AI 07019-20/AI/NIAID NIH HHS/United States
GR  - AI 34404/AI/NIAID NIH HHS/United States
GR  - CA 16885/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CXC)
RN  - 0 (E-Selectin)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lymphotoxin-alpha)
RN  - 0 (MADCAM1 protein, human)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Madcam1 protein, mouse)
RN  - 0 (Monokines)
RN  - 0 (Mucoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules/*biosynthesis/genetics
MH  - Chemokine CCL2/biosynthesis/genetics
MH  - Chemokine CCL4
MH  - Chemokine CCL5/biosynthesis/genetics
MH  - Chemokine CXCL10
MH  - Chemokine CXCL2
MH  - Chemokines/*biosynthesis/genetics
MH  - Chemokines, CXC/biosynthesis/genetics
MH  - Cytotoxicity, Immunologic
MH  - E-Selectin/biosynthesis/genetics
MH  - Embryonic and Fetal Development
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Immunoglobulins/biosynthesis/genetics
MH  - Inflammation/*physiopathology
MH  - Intercellular Adhesion Molecule-1/biosynthesis/genetics
MH  - Lymphoid Tissue/*embryology
MH  - Lymphotoxin-alpha/chemistry/*pharmacology/physiology
MH  - Macrophage Inflammatory Proteins/biosynthesis/genetics
MH  - Mice
MH  - Monokines/biosynthesis/genetics
MH  - Mucoproteins/biosynthesis/genetics
MH  - Recombinant Fusion Proteins/pharmacology
MH  - Species Specificity
MH  - Stimulation, Chemical
MH  - Tumor Cells, Cultured
MH  - Vascular Cell Adhesion Molecule-1/biosynthesis/genetics
EDAT- 1998/12/23 00:00
MHDA- 1998/12/23 00:01
CRDT- 1998/12/23 00:00
PHST- 1998/12/23 00:00 [pubmed]
PHST- 1998/12/23 00:01 [medline]
PHST- 1998/12/23 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1998 Dec 15;161(12):6853-60.