PMID- 9865788
OWN - NLM
STAT- MEDLINE
DCOM- 19990223
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 6
IP  - 6
DP  - 1998 Sep
TI  - Interspecies comparative genome hybridization and interspecies representational 
      difference analysis reveal gross DNA differences between humans and great apes.
PG  - 487-94
AB  - Comparative chromosome G-/R-banding, comparative gene mapping and chromosome 
      painting techniques have demonstrated that only few chromosomal rearrangements 
      occurred during great ape and human evolution. Interspecies comparative genome 
      hybridization (CGH), used here in this study, between human, gorilla and pygmy 
      chimpanzee revealed species-specific regions in all three species. In contrast to 
      the human, a far more complex distribution of species-specific blocks was 
      detected with CGH in gorilla and pygmy chimpanzee. Most of these blocks coincide 
      with already described heterochromatic regions on gorilla and chimpanzee 
      chromosomes. Representational difference analysis (RDA) was used to subtract the 
      complex genome of gorilla against human in order to enrich gorilla-specific DNA 
      sequences. Gorilla-specific clones isolated with this technique revealed a 32-bp 
      repeat unit. These clones were mapped by fluorescence in situ hybridization 
      (FISH) to the telomeric regions of gorilla chromosomes that had been shown by 
      interspecies CGH to contain species-specific sequences.
FAU - Toder, R
AU  - Toder R
AD  - Institute of Human Genetics and Anthropology, University of Freiburg, Germany. 
      rtoder@ukl.uni-freiburg.de
FAU - Xia, Y
AU  - Xia Y
FAU - Bausch, E
AU  - Bausch E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - DNA/*genetics
MH  - *Genome, Human
MH  - Gorilla gorilla/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Hybridization
MH  - Pan troglodytes/*genetics
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
EDAT- 1998/12/29 00:00
MHDA- 1998/12/29 00:01
CRDT- 1998/12/29 00:00
PHST- 1998/12/29 00:00 [pubmed]
PHST- 1998/12/29 00:01 [medline]
PHST- 1998/12/29 00:00 [entrez]
AID - 10.1023/a:1009208730186 [doi]
PST - ppublish
SO  - Chromosome Res. 1998 Sep;6(6):487-94. doi: 10.1023/a:1009208730186.