PMID- 9869381
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 719
IP  - 1-2
DP  - 1998 Nov 20
TI  - Determination of acrolein in human urine by headspace gas chromatography and mass 
      spectrometry.
PG  - 209-12
AB  - A rapid and sensitive headspace gas chromatographic and mass spectrometric 
      (GC-MS) method was developed for the determination of acrolein in human urine. A 
      0.5-ml urine sample in a glass vial containing propionaldehyde as an internal 
      standard was heated at 80 degrees C for 5 min. A 0.1-ml volume of headspace vapor 
      was injected into a GC-MS instrument. Acrolein and propionaldehyde were coeluted 
      at 3.1 min using a DB-1 capillary column, and well separated by selective ion 
      monitoring (SIM) mode using ions m/z 56.05 and m/z 58.05. The interassay and 
      intraassay coefficient of variation were 0.99% and 3.3%. The calibration curve 
      demonstrated a good linearity throughout concentrations ranging from 1 to 1000 
      nM. However, due to a wide variation of acrolein evaporation rates from human 
      urine, a calibration curve must be established for each urine specimen using a 
      standard addition method and detection limit varied from 1 to 5 nM. The total 
      analysis time for two samples from one urine specimen required about 15 min. 
      Therefore, this method is convenient for the urgent monitoring of urinary 
      acrolein in patients to whom alkylating agents are administered.
FAU - Sakura, N
AU  - Sakura N
AD  - Hiroshima University School of Medicine, Japan.
FAU - Nishimura, S
AU  - Nishimura S
FAU - Fujita, N
AU  - Fujita N
FAU - Namera, A
AU  - Namera A
FAU - Yashiki, M
AU  - Yashiki M
FAU - Kojima, T
AU  - Kojima T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 7864XYD3JJ (Acrolein)
SB  - IM
MH  - Acrolein/*urine
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 1998/12/30 00:00
MHDA- 1998/12/30 00:01
CRDT- 1998/12/30 00:00
PHST- 1998/12/30 00:00 [pubmed]
PHST- 1998/12/30 00:01 [medline]
PHST- 1998/12/30 00:00 [entrez]
AID - 10.1016/s0378-4347(98)00422-8 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1998 Nov 20;719(1-2):209-12. doi: 
      10.1016/s0378-4347(98)00422-8.