PMID- 9869391
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20190921
IS  - 0106-9543 (Print)
IS  - 0106-9543 (Linking)
VI  - 18
IP  - 6
DP  - 1998 Dec
TI  - Comparative study of 5' UTR and NS3R primers for the detection of GB virus 
      C/hepatitis G virus RNA in Japanese.
PG  - 378-82
AB  - AIMS/BACKGROUND: Many epidemiological studies of new hepatitis viruses, including 
      GB virus C (GBV-C) and hepatitis G virus (HGV), have used polymerase chain 
      reaction (PCR) primers designed for the third nonstructural region (NS3R). 
      However, a homology study of GBV-C and HGV genomes revealed that the 5' 
      untranslated region (5'UTR) was more conserved than NS3R. METHODS: We attempted 
      to detect GBV-C/HGV using PCR primers corresponding to the 5' UTR, and compared 
      its incidence to that derived from NS3R primers. Furthermore, PCR products 
      amplified using the 5' UTR primers were sequenced and subjected to phylogenetic 
      analysis. RESULTS: In patients with chronic hepatitis C, the prevalence of 
      GBV-C/HGV by PCR with the NS3R and 5' UTR primers was 5.1% (4/78) and 17.9% 
      (14/78), respectively, and in patients on hemodialysis, it was 0% (0/81) and 5.9% 
      (5/85), respectively. We could not detect GBV-C/HGV in patients with non-A-C 
      liver disease. The incidence of GBV-C/HGV by 5' UTR primers was higher than by 
      NS3R primers. After DNA sequencing at 5' UTR, phylogenetic analysis showed two 
      types of GBV-C/HGV, Jap and HGV types. CONCLUSION: 5' UTR primers proved highly 
      sensitive for detection of GBV-C/HGV and were superior to the NS3R primers.
FAU - Tanaka, H
AU  - Tanaka H
AD  - Third Department of Internal Medicine, Wakayama Medical College, Wakayama City, 
      Japan.
FAU - Miyano, M
AU  - Miyano M
FAU - Ueda, H
AU  - Ueda H
FAU - Doi, R
AU  - Doi R
FAU - Mimura, K
AU  - Mimura K
FAU - Nishide, I
AU  - Nishide I
FAU - Yukawa, S
AU  - Yukawa S
LA  - eng
SI  - GENBANK/AB015884
SI  - GENBANK/AB016034
SI  - GENBANK/AB016035
SI  - GENBANK/AB016036
SI  - GENBANK/AB016037
SI  - GENBANK/AB016038
SI  - GENBANK/AB016039
SI  - GENBANK/AB016040
PT  - Comparative Study
PT  - Journal Article
PL  - Denmark
TA  - Liver
JT  - Liver
JID - 8200939
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA Primers)
RN  - 0 (NS3 protein, hepatitis C virus)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - 5' Untranslated Regions/*genetics
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Base Sequence
MH  - DNA Primers/chemistry
MH  - Female
MH  - Flaviviridae/*genetics/isolation & purification
MH  - Hepatitis, Viral, Human/*diagnosis/epidemiology
MH  - Humans
MH  - Japan/epidemiology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/*analysis
MH  - Viral Nonstructural Proteins/*genetics/isolation & purification
EDAT- 1998/12/30 00:00
MHDA- 1998/12/30 00:01
CRDT- 1998/12/30 00:00
PHST- 1998/12/30 00:00 [pubmed]
PHST- 1998/12/30 00:01 [medline]
PHST- 1998/12/30 00:00 [entrez]
AID - 10.1111/j.1600-0676.1998.tb00821.x [doi]
PST - ppublish
SO  - Liver. 1998 Dec;18(6):378-82. doi: 10.1111/j.1600-0676.1998.tb00821.x.