PMID- 9870099
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 20
IP  - 6
DP  - 1998 Dec
TI  - Characterization of cosmids and telomeres in cytogenetic preparations by 3D 
      confocal fluorescence.
PG  - 477-82
AB  - OBJECTIVE: To analyze, with fluorescent probes, by three-dimensional (3D) 
      emission patterns, fluorescence in situ hybridization (FISH) preparations 
      (cosmids, telomeres) and to perform factor analysis of medical image sequences 
      (FAMIS); to use FISH to track relevant DNA sequences in cell nuclei during 
      interphase and in mitotic chromosomes; and to use cytogenetic techniques, 
      resulting in flat preparations of whole cells that are assumed to preserve probe 
      access to their targets. STUDY DESIGN: The study design entailed labeling targets 
      by probes (sequences labeled by fluorescein isothiocyanate) in nuclei and/or 
      chromosomes stained by propidium iodide. Visualization of targets was improved 
      when 3D sequences of images obtained on a single photomultiplier detector of the 
      confocal microscope by z stepping were investigated by FAMIS. RESULTS: Factors 
      and factor images showed that targets could be detected and differentiated in 
      focal planes inside nuclei or chromosomes. CONCLUSION: It is possible to localize 
      cosmids in cell nuclei at interphase and telomeres in mitotic chromosomes by 
      means of 3D sequences of images.
FAU - Kahn, E
AU  - Kahn E
AD  - Institut National de la Sante et de la Recherche Medicale U494, Centre 
      Hospitalier Universitaire Pitie-Salpetriere, Paris.
FAU - Philippe, C
AU  - Philippe C
FAU - Frouin, F
AU  - Frouin F
FAU - Di Paola, R
AU  - Di Paola R
FAU - Bernheim, A
AU  - Bernheim A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Cosmids/*ultrastructure
MH  - Factor Analysis, Statistical
MH  - Fluorescent Dyes
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Male
MH  - *Microscopy, Confocal
MH  - Mitosis
MH  - Telomere/*ultrastructure
EDAT- 1998/12/31 00:00
MHDA- 1998/12/31 00:01
CRDT- 1998/12/31 00:00
PHST- 1998/12/31 00:00 [pubmed]
PHST- 1998/12/31 00:01 [medline]
PHST- 1998/12/31 00:00 [entrez]
PST - ppublish
SO  - Anal Quant Cytol Histol. 1998 Dec;20(6):477-82.