PMID- 9870396
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20061115
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 19
IP  - 16-17
DP  - 1998 Nov
TI  - Screening for urinary amphetamine and analogs by capillary electrophoretic 
      immunoassays and confirmation by capillary electrophoresis with on-column 
      multiwavelength absorbance detection.
PG  - 2956-66
AB  - This paper characterizes competitive binding, electrokinetic capillary-based 
      immunoassays for screening of urinary amphetamine (A) and analogs using reagents 
      which were commercialized for a fluorescence polarization immunoassay (FPIA). 
      After incubation of 25 microL urine with the reactants, a small aliquot of the 
      mixture is applied onto a fused-silica capillary and unbound fluorescein-labeled 
      tracer compounds are monitored by capillary electrophoresis with on-column 
      laser-induced fluorescence detection. Configurations in presence and absence of 
      micelles were investigated and found to be capable of recognizing urinary 
      D-(+)-amphetamine at concentrations > about 80 ng/mL. Similar responses were 
      obtained for racemic methamphetamine (MA) and 3,4-methylenedioxymethamphetamine 
      (MDMA). The electrokinetic immunoassay data suggest that the FPIA reagent kit 
      includes two immunoassay systems (two antibodies and two tracer molecules), one 
      that recognizes MA and MDMA, and one that is geared towards monitoring of A. For 
      confirmation analysis of urinary amphetamines and ephedrines, capillary 
      electrophoresis in a pH 9.2 buffer and multiwavelength UV detection was employed. 
      The suitability of the electrokinetic methods for screening and confirmation is 
      demonstrated via analysis of patient and external quality control urines.
FAU - Ramseier, A
AU  - Ramseier A
AD  - Department of Clinical Pharmacology, University of Bern, Switzerland.
FAU - Caslavska, J
AU  - Caslavska J
FAU - Thormann, W
AU  - Thormann W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Amphetamines)
RN  - 0 (Central Nervous System Stimulants)
SB  - IM
MH  - Amphetamines/*urine
MH  - Central Nervous System Stimulants/*urine
MH  - Electrophoresis, Capillary
MH  - Fluorescence Polarization Immunoassay
MH  - Humans
MH  - Substance Abuse Detection/*methods
EDAT- 1998/12/31 00:00
MHDA- 1998/12/31 00:01
CRDT- 1998/12/31 00:00
PHST- 1998/12/31 00:00 [pubmed]
PHST- 1998/12/31 00:01 [medline]
PHST- 1998/12/31 00:00 [entrez]
AID - 10.1002/elps.1150191627 [doi]
PST - ppublish
SO  - Electrophoresis. 1998 Nov;19(16-17):2956-66. doi: 10.1002/elps.1150191627.