PMID- 987089
OWN - NLM
STAT- MEDLINE
DCOM- 19761121
LR  - 20190723
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 58
IP  - 3
DP  - 1976 Sep
TI  - A galactosidase immunosorbent test for human immunoglobulin E.
PG  - 426-31
AB  - We report here the development of a galactosidase-immunosorbent test (GIST) for 
      immunoglobulin E (IgE) antibodies in which the amount of galactosidase adsorbed 
      to a cellulose disc is a single valued function of IgE concentration in human 
      serum. Rabbit anti-IgE immunoglobulin insolubilized on cellulose discs is 
      incubated sequentially with human serum, sheep anti-IgE serum, and a covalent 
      conjugate of rabbit antisheep immunoglobulin with the enzyme beta-D-galactoside 
      galactohydrolase (E.C.) 3.2.1.23). Colorimetric assay of enzyme conjugate 
      adsorbed to discs permits quantitation of 1.0 to 25 ng of IgE per test. 
      Concentrations of IgE in 48 sera as measured by the GIST gave a linear 
      correlation coefficient of 0.97 with IgE concentrations as determined by 
      radioimmunoassay. Preliminary studies indicate that the GIST makes possible 
      nonisotopic measurement of ragweed-specific IgE antibiotics in human serum. The 
      GIST for IgE is simple to perform and requires neither short-lived radioisotopes, 
      expensive scintillation detection equipment, nor scarce, purified IgE.
FAU - Weltman, J K
AU  - Weltman JK
FAU - Frackelton, A R Jr
AU  - Frackelton AR Jr
FAU - Szaro, R P
AU  - Szaro RP
FAU - Rotman, B
AU  - Rotman B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.2.1.- (Galactosidases)
SB  - IM
MH  - Allergens
MH  - Animals
MH  - Binding Sites, Antibody
MH  - *Galactosidases
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Plants/immunology
MH  - Rabbits
MH  - Radioimmunoassay
EDAT- 1976/09/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1976/09/01 00:00
PHST- 1976/09/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1976/09/01 00:00 [entrez]
AID - 0091-6749(76)90123-8 [pii]
AID - 10.1016/0091-6749(76)90123-8 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 1976 Sep;58(3):426-31. doi: 10.1016/0091-6749(76)90123-8.