PMID- 9872029
OWN - NLM
STAT- MEDLINE
DCOM- 19990114
LR  - 20161013
IS  - 0929-6646 (Print)
IS  - 0929-6646 (Linking)
VI  - 97
IP  - 11
DP  - 1998 Nov
TI  - Polymorphisms of twelve short tandem repeat loci in a Taiwanese population and 
      their application in parentage testing.
PG  - 738-44
AB  - With the advancement of techniques in molecular biology, rapid, sensitive, and 
      reliable methods of DNA typing for parentage testing have become available. In 
      this study, we evaluated the usefulness of multiplex polymerase chain reaction 
      (PCR) with 12 unlinked short tandem repeat (STR) loci for paternity testing in 
      Taiwan. The genetic informativeness of this test was then compared with that of 
      conventional human leukocyte antigen (HLA) analysis in 167 parentage studies. The 
      12 STR loci alone provided a cumulative power of exclusion of up to 0.9998. 
      Paternity was excluded in 59 (35.3%) cases, including 40 of 112 paternity trios 
      and 19 of 55 paternity duos. In the 40 trios in which paternity was excluded, a 
      mean of 6 (range, 3-9) incompatible STR markers were in the 19 duos in which 
      paternity was excluded, a mean of 4 (range, 1-8) incompatible STR markers were 
      noted. In the 72 trios in which the alleged paternity could not be excluded, the 
      mean probabilities of paternity (PP) were 90.6863% with HLA testing alone, 
      99.9847% with STR analysis alone, and 99.9972% with combined HLA and STR 
      analysis. In the 36 duos in which the alleged paternity could not be excluded, 
      the mean PPs were 81.4768% with HLA testing alone, 99.6124% with STR analysis 
      alone, and 99.9145% with combined HLA and STR analysis. These results suggest 
      that STR analysis is very powerful when used alone for paternity trio testing and 
      when combined with conventional serologic HLA typing for duo parentage testing in 
      the Taiwan population.
FAU - Tzeng, C H
AU  - Tzeng CH
AD  - Section of Transfusion Medicine, Veterans General Hospital-Taipei, School of 
      Medicine, National Yang-Ming University, Taiwan.
FAU - Lyou, J Y
AU  - Lyou JY
FAU - Chen, Y R
AU  - Chen YR
FAU - Hu, H Y
AU  - Hu HY
FAU - Lin, J S
AU  - Lin JS
FAU - Yung, C H
AU  - Yung CH
FAU - Choo, K B
AU  - Choo KB
FAU - Wang, S Y
AU  - Wang SY
FAU - Lee, J C
AU  - Lee JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - J Formos Med Assoc
JT  - Journal of the Formosan Medical Association = Taiwan yi zhi
JID - 9214933
SB  - IM
MH  - *Chromosome Mapping
MH  - Female
MH  - Histocompatibility Testing
MH  - Humans
MH  - Male
MH  - *Paternity
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - *Tandem Repeat Sequences
EDAT- 1999/01/01 00:00
MHDA- 1999/01/01 00:01
CRDT- 1999/01/01 00:00
PHST- 1999/01/01 00:00 [pubmed]
PHST- 1999/01/01 00:01 [medline]
PHST- 1999/01/01 00:00 [entrez]
PST - ppublish
SO  - J Formos Med Assoc. 1998 Nov;97(11):738-44.