PMID- 9872665
OWN - NLM
STAT- MEDLINE
DCOM- 19990310
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 6
IP  - 5
DP  - 1998 Aug
TI  - Characterization of reciprocal translocations in pigs using dual-colour 
      chromosome painting and primed in situ DNA labelling.
PG  - 361-6
AB  - We report the use of dual-colour chromosome painting to determine the exact 
      nature of certain chromosome rearrangements observed in the pig (Sus scrofa 
      domestica). The chromosomal abnormalities were detected by GTG- and RBG-banding 
      techniques. The initially proposed interpretations were: (1) 
      rcp(6;13)(p1.5;q4.1); (2) rcp(11;16)(p1.4;q1.4); (3) rcp(6;16)(p1.1;q1.1); (4) 
      rcp(13;17)(q4.1;q1.1); (5) rcp(6;14)(q2.7;q2.1); (6) rcp(3;5)(p1.3;q2.3); (7) 
      rcp(2; 14)(q1.3;q2.7); (8) rcp(15;17)(q1.3;q2.1). Hybridizations were carried out 
      with biotin- and digoxigenin-labelled probes obtained by priming authorizing 
      random mismatches polymerase chain reaction (PARM-PCR) amplification of porcine 
      flow-sorted chromosomes. In some cases, i.e. (1), (4), (5), (6), (7) and (8), the 
      fluorescence in situ hybridization (FISH) results allowed confirmation of the 
      interpretations proposed with classical cytogenetic methods. Chromosome painting 
      proved the reciprocity of the translocation in cases (1), (6) and (8), whereas 
      modifications of the formula were proposed for case (2). Primed in situ DNA 
      labelling (PRINS) experiments have also been carried out in case (3) using a 
      primer specific for the centromeres of acrocentric chromosomes (first experiment) 
      or a primer specific for the centromeres of a subset of meta- and submetacentric 
      chromosomes including chromosome 6 (second experiment). It allowed us to 
      demonstrate that the breakpoints occurred in the centromeric region of chromosome 
      16 and in the p. arm of chromosome 6, just above the centromere.
FAU - Pinton, A
AU  - Pinton A
AD  - ENVT, Laboratoire de Cytogenetique, Toulouse, France.
FAU - Ducos, A
AU  - Ducos A
FAU - Seguela, A
AU  - Seguela A
FAU - Berland, H M
AU  - Berland HM
FAU - Darre, R
AU  - Darre R
FAU - Darre, A
AU  - Darre A
FAU - Pinton, P
AU  - Pinton P
FAU - Schmitz, A
AU  - Schmitz A
FAU - Cribiu, E P
AU  - Cribiu EP
FAU - Yerle, M
AU  - Yerle M
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Primers)
RN  - 6SO6U10H04 (Biotin)
RN  - 9007-49-2 (DNA)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
MH  - Animals
MH  - Biotin
MH  - *Chromosome Painting
MH  - Chromosomes/chemistry
MH  - DNA/analysis
MH  - *DNA Primers
MH  - Digoxigenin
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction/methods
MH  - Swine/*genetics
MH  - *Translocation, Genetic
EDAT- 1999/01/01 00:00
MHDA- 1999/01/01 00:01
CRDT- 1999/01/01 00:00
PHST- 1999/01/01 00:00 [pubmed]
PHST- 1999/01/01 00:01 [medline]
PHST- 1999/01/01 00:00 [entrez]
AID - 10.1023/a:1009244415357 [doi]
PST - ppublish
SO  - Chromosome Res. 1998 Aug;6(5):361-6. doi: 10.1023/a:1009244415357.