PMID- 9872667
OWN - NLM
STAT- MEDLINE
DCOM- 19990310
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 6
IP  - 5
DP  - 1998 Aug
TI  - XY sex reversal in the wood lemming is associated with deletion of Xp21-23 as 
      revealed by chromosome microdissection and fluorescence in situ hybridization.
PG  - 379-83
AB  - In the wood lemming (Myopus schisticolor), XY sex reversal occurs naturally 
      because of the presence of an X chromosome variant designated X*. The two types 
      of X chromosome, X and X*, can be distinguished by G-banding, and analyses have 
      demonstrated complex rearrangements of the short arm of X*. Here, chromosomal 
      microdissection, degenerate oligonucleotide-primed polymerase chain reaction 
      (DOP-PCR) and fluorescence in situ hybridization (FISH) techniques have been used 
      to generate and map DNA probes for different parts of the X and X* chromosomes. 
      The results showed that the region of Xp21-23 is deleted from the X* and some of 
      the deleted DNA sequences are homologous to the mouse gamma-satellite. The 
      deletion must be associated with the sex reversal in this species. FISH 
      experiments with dissected probes of X and distal half of Xq provided evidence 
      for presence of homologous sequences between large regions of the X and Y 
      chromosomes, including euchromatic and heterochromatic parts of the sex 
      chromosomes. The findings of this study will be of significance for further 
      cloning of important candidate gene(s) responsible for the XY sex reversal.
FAU - Liu, W S
AU  - Liu WS
AD  - Department of Genetics, Uppsala University, Sweden. wansheng.liu@genetik.uu.se
FAU - Eriksson, L
AU  - Eriksson L
FAU - Fredga, K
AU  - Fredga K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
SB  - IM
MH  - Animals
MH  - Arvicolinae/*genetics
MH  - *Disorders of Sex Development
MH  - Dissection/methods
MH  - *Gene Deletion
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Molecular Probe Techniques
MH  - Polymerase Chain Reaction/methods
MH  - Sex Chromosomes
MH  - *Sex Determination Processes
MH  - *X Chromosome
EDAT- 1999/01/01 00:00
MHDA- 1999/01/01 00:01
CRDT- 1999/01/01 00:00
PHST- 1999/01/01 00:00 [pubmed]
PHST- 1999/01/01 00:01 [medline]
PHST- 1999/01/01 00:00 [entrez]
AID - 10.1023/a:1009273205788 [doi]
PST - ppublish
SO  - Chromosome Res. 1998 Aug;6(5):379-83. doi: 10.1023/a:1009273205788.