PMID- 9872818
OWN - NLM
STAT- MEDLINE
DCOM- 19990211
LR  - 20061115
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 159
IP  - 1
DP  - 1999 Jan
TI  - Derivation of tumor-specific cytolytic T-cell clones from two lung cancer 
      patients with long survival.
PG  - 55-62
AB  - We derived lung carcinoma cell lines from tumor material resected from a patient 
      with small-cell lung cancer (SCLC) and from a patient with non-small-cell lung 
      cancer (NSCLC). The patient with NSCLC was vaccinated with irradiated autologous 
      tumor cells. The two patients enjoyed an exceptionally favorable clinical 
      evolution and are currently without signs of cancer 10 and 8 yr after their 
      diagnoses, respectively. Autologous mixed lymphocyte-tumor cell cultures (MLTC) 
      were produced with blood lymphocytes stimulated with irradiated autologous tumor 
      cells. The first patient's SCLC cells, which carried a small amount of human 
      leukocyte antigen (HLA) class I molecules, were incubated with interferon-gamma 
      (IFN-gamma) before being used as stimulator cells. A cytolytic T-lymphocyte (CTL) 
      clone was derived that specifically lysed the IFN-gamma-treated SCLC cells but 
      did not lyse untreated tumor cells or autologous lymphoblasts. Clones of 
      autologous tumor-specific CTL, directed against the NSCLC cells of the other 
      patient, were also obtained. These tumor cells carried a higher level of HLA 
      class I molecules and were lysed by the CTL without incubation with IFN-gamma. 
      Altogether, these results indicate that SCLC and NSCLC cancer cells can be 
      recognized by autologous CTL, and might therefore be susceptible to specific 
      immunotherapy.
FAU - Weynants, P
AU  - Weynants P
AD  - Ludwig Institute for Cancer Research, Brussels, Services de Pneumologie et 
      d'Anatomopathologie, Hopital de Mont-Godinne, Universite Catholique de Louvain, 
      Yvoir; and Cellular Genetics Unit, Universite Catholique de Louvain, 
      Brussels,Belgium.
FAU - Thonnard, J
AU  - Thonnard J
FAU - Marchand, M
AU  - Marchand M
FAU - Delos, M
AU  - Delos M
FAU - Boon, T
AU  - Boon T
FAU - Coulie, P G
AU  - Coulie PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/immunology/*pathology/physiopathology
MH  - Carcinoma, Small Cell/immunology/*pathology/physiopathology
MH  - Clone Cells
MH  - Humans
MH  - Immunologic Memory/physiology
MH  - Lung Neoplasms/immunology/*pathology/physiopathology
MH  - Lymphocytes, Tumor-Infiltrating/immunology/*pathology
MH  - Male
MH  - Middle Aged
MH  - Survival Analysis
MH  - T-Lymphocytes, Cytotoxic/immunology/*pathology
MH  - Tumor Cells, Cultured
EDAT- 1999/01/05 00:00
MHDA- 1999/01/05 00:01
CRDT- 1999/01/05 00:00
PHST- 1999/01/05 00:00 [pubmed]
PHST- 1999/01/05 00:01 [medline]
PHST- 1999/01/05 00:00 [entrez]
AID - 10.1164/ajrccm.159.1.9805073 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 1999 Jan;159(1):55-62. doi: 
      10.1164/ajrccm.159.1.9805073.